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- W2022134269 abstract "The effect of different pH of resuspension media on the viability of hepatocytes preserved (for 96 h at 4°C) in University of Wisconsin solution (UW solution) was analyzed. After this cold resuspension media storage, we evaluated the rewarming step (incubation time 120 min at 37°C) using different pH levels (6.80, 7.00, 7.20, and 7.40). Cell viability assessed by trypan blue exclusion (TBE) showed a significant difference (p < 0.05) for cells incubated at pH = 7.20. For instance, TBE expressed as percent of change was 78.1 ± 1.4 compared with cells tested at other pH (pH = 6.80, TBE = 44.2 ±9.5; pH = 7.00, TBE = 66.5 ±1.1 and pH = 7.40, TBE = 62.0 ± 1.4). We also evaluated the capacity of these cells both to maintain potassium content (0.509± 0.230 μEq). K+/106 cells) and to synthesize urea (5.36 ± 1.81μmol Urea/106 cells). These results were compared with those obtained from freshly isolated non preserved hepatocytes (0.518 ± 0.060 μEq. K+/106 cells and 5.91 ± 0.43 μmol Urea/106 cells). The results show that viability is pH dependent and suggest that when resuspension media were used, the viability of hepatocytes was improved after 96 h of cold storage." @default.
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- W2022134269 title "Importance of pH in resuspension media on viability of hepatocytes preserved in University of Wisconsin solution" @default.
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- W2022134269 doi "https://doi.org/10.1016/0963-6897(94)00079-y" @default.
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