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• W2022142539 endingPage "1290" @default.
• W2022142539 startingPage "1285" @default.
• W2022142539 abstract "Exceptionally high mass resolving power and mass accuracy combined with tandem mass spectrometry (MSn) capability make Fourier transform ion cyclotron resonance mass spectrometry a powerful tool for structure verification and determination of biological macromolecules. By means of local internal calibration and electron mass correction, mass accuracy better than ±0.5 ppm was achieved for two oligosaccharide antibiotics, Saccharomicins A and B, consistent with the proposed elemental compositions based upon NMR data. High resolution and high mass accuracy MS/MS data were obtained for both oligosaccharides by use of infrared multiphoton dissociation (IRMPD) with a 40 W continuous-wave CO2 laser. The spectra were charge-state deconvolved by the “Z-score” algorithm to yield much simpler mass-only spectra. Sequences of 15 sugar residues could be confirmed from the charge state deconvolved accurate mass MS/MS spectra for Saccharomicins A and B, even without use of traditional prior permethylation. A fragment corresponding to an internal sugar loss rearrangement was observed by IRMPD and studied by collision activated dissociation MS4." @default.
• W2022142539 created "2016-06-24" @default.
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• W2022142539 date "1999-12-01" @default.
• W2022142539 modified "2023-10-16" @default.
• W2022142539 title "Structural validation of saccharomicins by high resolution and high mass accuracy fourier transform-ion cyclotron resonance-mass spectrometry and infrared multiphoton dissociation tandem mass spectrometry" @default.
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• W2022142539 doi "https://doi.org/10.1016/s1044-0305(99)00108-7" @default.
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