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- W2022144937 abstract "Preparation of heteroduplexes in large quantities with high purity is essential for the measurement of DNA mismatch repair (MMR) activity. Here we report a rapid, less labor-intensive method for the preparation of a heteroduplex plasmid that expresses the enhanced green fluorescent protein (EGFP) if the mismatch is repaired correctly. The method involves the use of a wild-type and a mutated EGFP expression plasmid and a few steps of enzymatic digestion. When the constructed heteroduplex EGFP plasmid was transfected into MMR-proficient and -deficient cell lines, the number of EGFP-expressing cells was much higher in the MMR-proficient cells than in the MMR-deficient cells, suggesting that the heteroduplex can be used for MMR activity assay in live model systems." @default.
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- W2022144937 date "2009-05-01" @default.
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- W2022144937 title "Preparation of heteroduplex enhanced green fluorescent protein plasmid for in vivo mismatch repair activity assay" @default.
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- W2022144937 doi "https://doi.org/10.1016/j.ab.2009.02.020" @default.
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