Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022145456> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2022145456 endingPage "265" @default.
- W2022145456 startingPage "247" @default.
- W2022145456 abstract "Two sublethal responses were used to investigate the effects of genotoxic metals on embryos of the purple sea urchin, Strongylocentrotus purpuratus. In addition to the standard measurement of developmental success, we used a novel genotoxic response, DNA-protein crosslink (DPC) induction, to assess the effects of embryo exposure to pentavalent arsenate (As), nickel (Ni) and hexavalent chromate (Cr). The procedure for sea urchin embryo DPC measurement was adapted from a mammalian cell assay using potassium-SDS precipitation and a DNA fluorochrome to quantify relative amounts of free and protein-bound DNA. Developmental abnormality and DPCs increased after a 48-h exposure to each of the three metals. Lowest observable effect concentrations (LOECs) for development were 0.011 mg l−1 As, 0.40 mg l−1 Ni, and 2.5 mg l−1 Cr. LOECs calculated for the DPC response to these same three metals were 0.023, 8.0, and 10.0 mg l−1, respectively. DPCs were transiently high in control embryos through the blastula stage, which is prior to the dramatic increase in transcription of the embryonic genome. By the gastrula and prism stages (subsequent to embryo gene transcription), there was a significant decrease in DPCs. Ni-exposed embryos exhibited the greatest magnitude of adverse effect in embryos exposed through the blastula stage, as compared to those exposed from blastula through late gastrula stage. We hypothesize that stage-sensitivity to Ni in sea urchin embryos may be related to the induction of persistent DPCs, and the prevention of normal transcription of the embryonic genome." @default.
- W2022145456 created "2016-06-24" @default.
- W2022145456 creator A5032827876 @default.
- W2022145456 creator A5035170452 @default.
- W2022145456 creator A5044014968 @default.
- W2022145456 date "1997-11-01" @default.
- W2022145456 modified "2023-10-18" @default.
- W2022145456 title "Developmental abnormalities and DNA-protein crosslinks in sea urchin embryos exposed to three metals" @default.
- W2022145456 cites W1643297181 @default.
- W2022145456 cites W1968794709 @default.
- W2022145456 cites W1970709762 @default.
- W2022145456 cites W1971925073 @default.
- W2022145456 cites W1974239718 @default.
- W2022145456 cites W1974508730 @default.
- W2022145456 cites W1980067770 @default.
- W2022145456 cites W1985682749 @default.
- W2022145456 cites W1988515903 @default.
- W2022145456 cites W1993831920 @default.
- W2022145456 cites W1997011319 @default.
- W2022145456 cites W2004035563 @default.
- W2022145456 cites W2011474602 @default.
- W2022145456 cites W2013881444 @default.
- W2022145456 cites W2015585025 @default.
- W2022145456 cites W2016443009 @default.
- W2022145456 cites W2017027282 @default.
- W2022145456 cites W2027885635 @default.
- W2022145456 cites W2035636241 @default.
- W2022145456 cites W2057778088 @default.
- W2022145456 cites W2062878518 @default.
- W2022145456 cites W2068779520 @default.
- W2022145456 cites W2069245006 @default.
- W2022145456 cites W211266491 @default.
- W2022145456 cites W2113046040 @default.
- W2022145456 cites W2113655681 @default.
- W2022145456 cites W2118497546 @default.
- W2022145456 cites W2134200987 @default.
- W2022145456 cites W2137269557 @default.
- W2022145456 cites W2142853872 @default.
- W2022145456 cites W2166778877 @default.
- W2022145456 doi "https://doi.org/10.1016/s0166-445x(97)00033-7" @default.
- W2022145456 hasPublicationYear "1997" @default.
- W2022145456 type Work @default.
- W2022145456 sameAs 2022145456 @default.
- W2022145456 citedByCount "16" @default.
- W2022145456 countsByYear W20221454562012 @default.
- W2022145456 countsByYear W20221454562020 @default.
- W2022145456 countsByYear W20221454562021 @default.
- W2022145456 countsByYear W20221454562022 @default.
- W2022145456 countsByYear W20221454562023 @default.
- W2022145456 crossrefType "journal-article" @default.
- W2022145456 hasAuthorship W2022145456A5032827876 @default.
- W2022145456 hasAuthorship W2022145456A5035170452 @default.
- W2022145456 hasAuthorship W2022145456A5044014968 @default.
- W2022145456 hasConcept C129536746 @default.
- W2022145456 hasConcept C153911025 @default.
- W2022145456 hasConcept C16685009 @default.
- W2022145456 hasConcept C196843134 @default.
- W2022145456 hasConcept C2776908312 @default.
- W2022145456 hasConcept C2777569064 @default.
- W2022145456 hasConcept C6047887 @default.
- W2022145456 hasConcept C68760520 @default.
- W2022145456 hasConcept C71924100 @default.
- W2022145456 hasConcept C86803240 @default.
- W2022145456 hasConcept C87073359 @default.
- W2022145456 hasConcept C95444343 @default.
- W2022145456 hasConceptScore W2022145456C129536746 @default.
- W2022145456 hasConceptScore W2022145456C153911025 @default.
- W2022145456 hasConceptScore W2022145456C16685009 @default.
- W2022145456 hasConceptScore W2022145456C196843134 @default.
- W2022145456 hasConceptScore W2022145456C2776908312 @default.
- W2022145456 hasConceptScore W2022145456C2777569064 @default.
- W2022145456 hasConceptScore W2022145456C6047887 @default.
- W2022145456 hasConceptScore W2022145456C68760520 @default.
- W2022145456 hasConceptScore W2022145456C71924100 @default.
- W2022145456 hasConceptScore W2022145456C86803240 @default.
- W2022145456 hasConceptScore W2022145456C87073359 @default.
- W2022145456 hasConceptScore W2022145456C95444343 @default.
- W2022145456 hasIssue "3-4" @default.
- W2022145456 hasLocation W20221454561 @default.
- W2022145456 hasOpenAccess W2022145456 @default.
- W2022145456 hasPrimaryLocation W20221454561 @default.
- W2022145456 hasRelatedWork W2005208118 @default.
- W2022145456 hasRelatedWork W2012048960 @default.
- W2022145456 hasRelatedWork W2021506754 @default.
- W2022145456 hasRelatedWork W2055526599 @default.
- W2022145456 hasRelatedWork W2084667467 @default.
- W2022145456 hasRelatedWork W2374832211 @default.
- W2022145456 hasRelatedWork W2391031927 @default.
- W2022145456 hasRelatedWork W2398558632 @default.
- W2022145456 hasRelatedWork W2784731000 @default.
- W2022145456 hasRelatedWork W2944989222 @default.
- W2022145456 hasVolume "39" @default.
- W2022145456 isParatext "false" @default.
- W2022145456 isRetracted "false" @default.
- W2022145456 magId "2022145456" @default.
- W2022145456 workType "article" @default.