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- W2022145629 abstract "The general secretory (Sec) pathway is the main pathway for protein transport. Sortase A has been shown to anchor surface protein containing an LPXTG motif to the cell wall. In order to know the distribution pattern of the Sec translocons and sortase A in Streptococcus mutans, we explored the subcellular localisation of SecA and sortase A. The anti-SecA antibody and anti-sortase A antibody were examined on Western blots to evaluate specificities. An indirect post-embedding immunogold method was used to determine the subcellular localisation of the Sec pathway and sortase A enzyme in S. mutans membrane. Immunoblotting experiments showed that the anti-SecA antibody and anti-sortase A antibody specifically recognised a single band of about 95 kDa and 27 kDa, respectively in the S. mutans GS-5 cell lysates. Immunogold electron micrographs showed that small and large gold particles clustered together at a distinct microdomain. These data showed for the first time that Sec translocase and sortase A colocalised to a single locus in the cytoplasmic membrane of S. mutans GS-5." @default.
- W2022145629 created "2016-06-24" @default.
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- W2022145629 date "2008-02-01" @default.
- W2022145629 modified "2023-10-18" @default.
- W2022145629 title "Sec translocase and sortase A are colocalised in a locus in the cytoplasmic membrane of Streptococcus mutans" @default.
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- W2022145629 doi "https://doi.org/10.1016/j.archoralbio.2007.08.008" @default.
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