Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022151883> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2022151883 endingPage "19" @default.
- W2022151883 startingPage "7" @default.
- W2022151883 abstract "We study the uptake of amitriptyline, which is a common cause of overdose-related fatalities, in aqueous solutions by 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes and liposomes composed of a mixture of DMPC and 1,2-dioleoyl-sn-glycero-3-[phospho-rac(1-glycerol)] (DOPG) lipids. The effect of drug concentration, liposomal charge, pH, salt, and protein presence on the drug uptake is investigated using two different methodologies, a precipitation and a centrifugation method. Furthermore, the time scale of the drug uptake is studied through qualitative observations at high pH and through conductivity measurements at neutral pH and found to be <5 s. The results of the quantitative studies show that the fractional drug uptake decreases with increasing drug concentration, and for a given concentration it increases with the pH and decreases in the presence of salt. We find that a larger amount of drug is sequestered by negatively charged liposomes (those containing DOPG) than liposomes with no net charge (DMPC). We speculate that the mechanism of drug uptake is due to both electrostatic interactions as well as hydrophobic effects. The fractional uptake by DMPC:DOPG in a 70:30 ratio is as high as 95% in water and about 90% in physiological buffer. The fractional uptake is also measured in presence of 2% (w/w) bovine serum albumin (BSA), which is approximately the protein concentration in the intercellular fluid. In presence of protein the fractional uptakes by 70:30 DMPC:DOPG liposomes and 50:50 DMPC:DOPG liposomes are 82 and 90%, respectively, at 125 muM drug amitriptyline. In the absence of liposomes, 67% of the drug is taken up by the protein in a 2% (w/w) BSA, 125 muM amitriptyline solution. Thus, addition of 50:50 DMPC:DOPG liposomes reduces the free drug concentration by a factor of about 3.5, making them attractive candidates for drug detoxification." @default.
- W2022151883 created "2016-06-24" @default.
- W2022151883 creator A5032289092 @default.
- W2022151883 creator A5033994808 @default.
- W2022151883 date "2006-08-01" @default.
- W2022151883 modified "2023-09-23" @default.
- W2022151883 title "Sequestration of amitriptyline by liposomes" @default.
- W2022151883 cites W1967431109 @default.
- W2022151883 cites W1994170152 @default.
- W2022151883 cites W2029714755 @default.
- W2022151883 cites W2061546525 @default.
- W2022151883 cites W2063127400 @default.
- W2022151883 cites W2070551974 @default.
- W2022151883 cites W2081499511 @default.
- W2022151883 cites W2119784812 @default.
- W2022151883 cites W2130433105 @default.
- W2022151883 cites W4213030902 @default.
- W2022151883 doi "https://doi.org/10.1016/j.jcis.2006.03.065" @default.
- W2022151883 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16643936" @default.
- W2022151883 hasPublicationYear "2006" @default.
- W2022151883 type Work @default.
- W2022151883 sameAs 2022151883 @default.
- W2022151883 citedByCount "28" @default.
- W2022151883 countsByYear W20221518832012 @default.
- W2022151883 countsByYear W20221518832013 @default.
- W2022151883 countsByYear W20221518832014 @default.
- W2022151883 countsByYear W20221518832015 @default.
- W2022151883 countsByYear W20221518832016 @default.
- W2022151883 countsByYear W20221518832023 @default.
- W2022151883 crossrefType "journal-article" @default.
- W2022151883 hasAuthorship W2022151883A5032289092 @default.
- W2022151883 hasAuthorship W2022151883A5033994808 @default.
- W2022151883 hasConcept C13245373 @default.
- W2022151883 hasConcept C178790620 @default.
- W2022151883 hasConcept C185154212 @default.
- W2022151883 hasConcept C185592680 @default.
- W2022151883 hasConcept C2776371256 @default.
- W2022151883 hasConcept C2779820397 @default.
- W2022151883 hasConcept C2780035454 @default.
- W2022151883 hasConcept C43617362 @default.
- W2022151883 hasConcept C55493867 @default.
- W2022151883 hasConcept C71924100 @default.
- W2022151883 hasConcept C7623868 @default.
- W2022151883 hasConcept C86807702 @default.
- W2022151883 hasConcept C98274493 @default.
- W2022151883 hasConceptScore W2022151883C13245373 @default.
- W2022151883 hasConceptScore W2022151883C178790620 @default.
- W2022151883 hasConceptScore W2022151883C185154212 @default.
- W2022151883 hasConceptScore W2022151883C185592680 @default.
- W2022151883 hasConceptScore W2022151883C2776371256 @default.
- W2022151883 hasConceptScore W2022151883C2779820397 @default.
- W2022151883 hasConceptScore W2022151883C2780035454 @default.
- W2022151883 hasConceptScore W2022151883C43617362 @default.
- W2022151883 hasConceptScore W2022151883C55493867 @default.
- W2022151883 hasConceptScore W2022151883C71924100 @default.
- W2022151883 hasConceptScore W2022151883C7623868 @default.
- W2022151883 hasConceptScore W2022151883C86807702 @default.
- W2022151883 hasConceptScore W2022151883C98274493 @default.
- W2022151883 hasIssue "1" @default.
- W2022151883 hasLocation W20221518831 @default.
- W2022151883 hasLocation W20221518832 @default.
- W2022151883 hasOpenAccess W2022151883 @default.
- W2022151883 hasPrimaryLocation W20221518831 @default.
- W2022151883 hasRelatedWork W1540707417 @default.
- W2022151883 hasRelatedWork W2043136003 @default.
- W2022151883 hasRelatedWork W2260748129 @default.
- W2022151883 hasRelatedWork W2392582611 @default.
- W2022151883 hasRelatedWork W2506563335 @default.
- W2022151883 hasRelatedWork W2892152679 @default.
- W2022151883 hasRelatedWork W2905111812 @default.
- W2022151883 hasRelatedWork W2948450196 @default.
- W2022151883 hasRelatedWork W2952743768 @default.
- W2022151883 hasRelatedWork W4303182829 @default.
- W2022151883 hasVolume "300" @default.
- W2022151883 isParatext "false" @default.
- W2022151883 isRetracted "false" @default.
- W2022151883 magId "2022151883" @default.
- W2022151883 workType "article" @default.