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• W2022154328 abstract "Introduction: The lesser pathogenicity of HIV-2 relative to HIV-1 is generally attributed to its slower replication. To compare the amounts of total HIV DNA during human HIV-1 and HIV-2 infection, we developed a quantitative real-time PCR method with a unique external quantification standard based on a single plasmid harboring both the HIV-1 and the HIV-2 LTR. Methods: Viral DNA load was compared between 40 HIV-1-infected and 42 HIV-2-infected antiretroviral-naive patients. Results: The difference between HIV-1 and HIV-2 proviral DNA load was highly significant in patients with CD4 cell counts > 500 cells/μl [HIV-1: n = 14; median, 2.5; interquartile range (IQR), 2.1–2.7; HIV-2: n = 22, median, 1.6; IQR, 1.0–2.0] and in patients with CD4 cell counts between 300 cells/μl and 500 cells/μl (HIV-1: n = 12; median, 2.7; IQR, 2.3–2.8; HIV-2: n = 11; median, 2.0; IQR, 1.0–2.4). Too few HIV-2-infected patients had CD4 cell counts < 300 cells/μl to detect a significant difference but DNA values were again lower in HIV-2-infected patients (HIV-1: n = 14; median, 2.9; IQR, 2.2–3.2; HIV-2: n = 9; median, 2.7; IQR, 2.2–3.3). Conclusions: These differences are in line with the natural histories of the two infections and show that HIV-2 infection is a valid model for studying the pathophysiology of HIV infection in general." @default.
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• W2022154328 date "2008-01-11" @default.
• W2022154328 modified "2023-10-18" @default.
• W2022154328 title "Differences in proviral DNA load between HIV-1- and HIV-2-infected patients" @default.
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• W2022154328 doi "https://doi.org/10.1097/qad.0b013e3282f42429" @default.
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