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- W2022156512 abstract "The structural gene for NAD+-dependent 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) from Pseudomonas putida E23 was cloned in Escherichia coli cells to obtain a large amount of the enzyme and its nucleotides were sequenced to study its structural relationship with other proteins. The gene encoded a polypeptide containing 295 amino acid residues and was in a cluster with the gene for methylmalonate semialdehyde dehydrogenase. Transformed E. coli cells overproduced 3-hydroxyisobutyrate dehydrogenase, and the recombinant enzyme was purified to homogeneity with a high yield. Lysine and asparagine residues, which are important in catalysis of the 3-hydroxyacid dehydrogenase family, are conserved in this enzyme." @default.
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- W2022156512 date "2003-01-01" @default.
- W2022156512 modified "2023-10-17" @default.
- W2022156512 title "Cloning and Overexpression of the 3-Hydroxyisobutyrate Dehydrogenase Gene fromPseudomonas putidaE23" @default.
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- W2022156512 doi "https://doi.org/10.1271/bbb.67.438" @default.
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