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• W2022156928 abstract "Effective discrimination of non-complementary nucleotides is an important factor to ensure the accuracy of hybridization-based nucleic acid analyses. The current study investigates the effects of the chemical nature, the positions, the numbers, and the cooperative behavior of mismatches as well as insertions on 20-mer and 30-mer duplexes. We observed the hybridization stability trend affected by mismatches: G:T approximately G:G > G:A > A:A approximately T:T > A:C approximately T:C > C:C. The experimental data show that mismatches at the center of the oligonucleotide probes have a more profound destabilizing effect on the hybridization stability than those at either ends. Insertions also demonstrate a similar destabilizing effect as mismatches. These results provide useful information for designing DNA microarray nucleotide probes and for improving the discrimination accuracy of hybridization-based detections." @default.
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• W2022156928 date "2008-11-28" @default.
• W2022156928 modified "2023-09-27" @default.
• W2022156928 title "Effects of mismatches and insertions on discrimination accuracy of nucleic acid probes." @default.
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• W2022156928 doi "https://doi.org/10.18388/abp.2008_3032" @default.
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