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- W2022161854 abstract "In this paper, we developed transfection of a single fluorescent nanosensor into a cell nucleus using step-wise fusion of liposome for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using Layer-by-Layer formation. The sensor is manipulated by optical tweezers and transfected into the cell or cell nucleus by membrane fusion. To achieve this, a nanotechnology was developed that creates a multi lamellar liposome, which we refer to as Layer-by-Layer absorbed lipid membrane (LBL Lipsome). We also evaluated measurement size and surface charge of multi-layered liposome by the method of resistive pulse sensing. We demonstrated the fabrication of the liposome containing single sensor, immobilization of the sensor to cellular membrane, and transfection of the sensor into the cell cytoplasm and nucleus." @default.
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- W2022161854 date "2013-08-01" @default.
- W2022161854 modified "2023-09-24" @default.
- W2022161854 title "Multi-layered liposome containing nanosensor for transfecting into a cell nucleus" @default.
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- W2022161854 doi "https://doi.org/10.1109/nano.2013.6721022" @default.
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