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- W2022161923 abstract "For the location of the aminoglycoside-(3)-N-acetyltransferase isoenzyme II (AAC(3)-II) in the bacterial cell, two strains were studied: Escherichia coli HB101(pJV03), producing the 31-kDa AAC (3)-II enzyme, and E. coli HB101, which served as a control. From each strain five protein fractions were prepared: culture supernatant, and proteins occurring in the periplasm, cytoplasm, inner membrane and outer membrane. All fractions were tested for enzymatic activity of AAC(3)-II. Most of the acetylating activity was found in the cytoplasmic fraction. The distribution of marker enzymes showed a good separation between the periplasmic and the cytoplasmic fraction." @default.
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- W2022161923 date "1991-06-01" @default.
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- W2022161923 title "Localisation of the aminoglycoside-(3)-N-acetyltransferase isoenzyme II inEscherichia coli" @default.
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- W2022161923 doi "https://doi.org/10.1111/j.1574-6968.1991.tb04720.x" @default.
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