FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022169135> ?p ?o ?g. }

• W2022169135 abstract "Alternative splicing of mouse beta3-adrenoceptor transcripts produces an additional receptor isoform (beta3b-adrenoceptor) with a C terminus comprising 17 amino acids distinct from the 13 in the known receptor (beta3a-adrenoceptor). We have shown that the beta3b-adrenoceptor couples to both Gs and Gi, whereas the beta3a-adrenoceptor couples only to Gs. To define the regions involved in this differential G protein coupling, we have compared wild-type, truncated, and mutant beta3-adrenoceptors. In Chinese hamster ovary cells expressing beta3-adrenoceptors truncated at the splicing point, cAMP accumulation with CL316243 [(R,R)-5-[2-[[2-(3-chlorophenyl)-2-hydroxyethyl]-amino]-propyl]1,3-benzodioxole-2,2-dicarboxylate] increased by 59% following pretreatment with pertussis toxin, suggesting that the C-terminal region of the beta3a-adrenoceptor inhibits coupling to Gi. We next utilized the cell-penetrating peptide Transportan 10 (Tp10) to introduce peptides comprising the different C-terminal tail fragments into cells expressing beta3a-adrenoceptor, beta3b-adrenoceptor, and the truncated beta3-adrenoceptor. Treatment with beta3a-Tp10 (1 microM) caused cAMP responses to CL316243 in the beta3a-adrenoceptor to become pertussis toxin-sensitive and display a 30% increase over control, whereas the other peptides did not affect any receptor. Mutation at a potential tyrosine phosphorylation site (Tyr392Ala beta3a-adrenoceptor) did not alter responses or pertussis toxin sensitivity relative to the parent receptor. Surprisingly, a Ser388Ala/Ser389Ala mutant beta3b-adrenoceptor became unresponsive to CL316243 while retaining an extracellular acidification rate response to SR59230A [3-(2-ethylphenoxy)-1-[(1,S)-1,2,3,4-tetrahydronapth-1-ylamino]-2S-2-propanol oxalate]. Our findings suggest that the beta3a-adrenoceptor cannot couple to Gi because of conformational changes induced by a protein(s) that interacts with residues in the C-terminal tail or because this protein(s) affects the intracellular localization of the beta3a-adrenoceptor." @default.
• W2022169135 created "2016-06-24" @default.
• W2022169135 creator A5023365025 @default.
• W2022169135 creator A5031608824 @default.
• W2022169135 creator A5044821695 @default.
• W2022169135 creator A5055703629 @default.
• W2022169135 creator A5057501316 @default.
• W2022169135 creator A5062218095 @default.
• W2022169135 creator A5087640455 @default.
• W2022169135 creator A5090259855 @default.
• W2022169135 date "2005-09-06" @default.
• W2022169135 modified "2023-09-27" @default.
• W2022169135 title "Functional Domains of the Mouse β3-Adrenoceptor Associated with Differential G Protein Coupling" @default.
• W2022169135 cites W1501986797 @default.
• W2022169135 cites W1517879640 @default.
• W2022169135 cites W1524561975 @default.
• W2022169135 cites W1574601702 @default.
• W2022169135 cites W1971552869 @default.
• W2022169135 cites W1988858269 @default.
• W2022169135 cites W1994969848 @default.
• W2022169135 cites W2000052819 @default.
• W2022169135 cites W2003234447 @default.
• W2022169135 cites W2011914502 @default.
• W2022169135 cites W2017046702 @default.
• W2022169135 cites W2021565075 @default.
• W2022169135 cites W2024040954 @default.
• W2022169135 cites W2026137267 @default.
• W2022169135 cites W2037174652 @default.
• W2022169135 cites W2038163496 @default.
• W2022169135 cites W2041843731 @default.
• W2022169135 cites W2057870351 @default.
• W2022169135 cites W2071667726 @default.
• W2022169135 cites W2077521702 @default.
• W2022169135 cites W2078721878 @default.
• W2022169135 cites W2080039276 @default.
• W2022169135 cites W2081688069 @default.
• W2022169135 cites W2083220635 @default.
• W2022169135 cites W2088812635 @default.
• W2022169135 cites W2090455072 @default.
• W2022169135 cites W2091659381 @default.
• W2022169135 cites W2131713441 @default.
• W2022169135 cites W2170678561 @default.
• W2022169135 cites W2336864235 @default.
• W2022169135 cites W67730575 @default.
• W2022169135 doi "https://doi.org/10.1124/jpet.105.091736" @default.
• W2022169135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16144970" @default.
• W2022169135 hasPublicationYear "2005" @default.
• W2022169135 type Work @default.
• W2022169135 sameAs 2022169135 @default.
• W2022169135 citedByCount "29" @default.
• W2022169135 countsByYear W20221691352012 @default.
• W2022169135 countsByYear W20221691352013 @default.
• W2022169135 countsByYear W20221691352015 @default.
• W2022169135 countsByYear W20221691352016 @default.
• W2022169135 countsByYear W20221691352017 @default.
• W2022169135 countsByYear W20221691352018 @default.
• W2022169135 countsByYear W20221691352019 @default.
• W2022169135 countsByYear W20221691352020 @default.
• W2022169135 crossrefType "journal-article" @default.
• W2022169135 hasAuthorship W2022169135A5023365025 @default.
• W2022169135 hasAuthorship W2022169135A5031608824 @default.
• W2022169135 hasAuthorship W2022169135A5044821695 @default.
• W2022169135 hasAuthorship W2022169135A5055703629 @default.
• W2022169135 hasAuthorship W2022169135A5057501316 @default.
• W2022169135 hasAuthorship W2022169135A5062218095 @default.
• W2022169135 hasAuthorship W2022169135A5087640455 @default.
• W2022169135 hasAuthorship W2022169135A5090259855 @default.
• W2022169135 hasConcept C104317684 @default.
• W2022169135 hasConcept C143065580 @default.
• W2022169135 hasConcept C153911025 @default.
• W2022169135 hasConcept C170493617 @default.
• W2022169135 hasConcept C175656101 @default.
• W2022169135 hasConcept C40465926 @default.
• W2022169135 hasConcept C49773422 @default.
• W2022169135 hasConcept C55493867 @default.
• W2022169135 hasConcept C80631254 @default.
• W2022169135 hasConcept C86803240 @default.
• W2022169135 hasConcept C95444343 @default.
• W2022169135 hasConceptScore W2022169135C104317684 @default.
• W2022169135 hasConceptScore W2022169135C143065580 @default.
• W2022169135 hasConceptScore W2022169135C153911025 @default.
• W2022169135 hasConceptScore W2022169135C170493617 @default.
• W2022169135 hasConceptScore W2022169135C175656101 @default.
• W2022169135 hasConceptScore W2022169135C40465926 @default.
• W2022169135 hasConceptScore W2022169135C49773422 @default.
• W2022169135 hasConceptScore W2022169135C55493867 @default.
• W2022169135 hasConceptScore W2022169135C80631254 @default.
• W2022169135 hasConceptScore W2022169135C86803240 @default.
• W2022169135 hasConceptScore W2022169135C95444343 @default.
• W2022169135 hasLocation W20221691351 @default.
• W2022169135 hasLocation W20221691352 @default.
• W2022169135 hasOpenAccess W2022169135 @default.
• W2022169135 hasPrimaryLocation W20221691351 @default.
• W2022169135 hasRelatedWork W1504083190 @default.
• W2022169135 hasRelatedWork W1561695981 @default.
• W2022169135 hasRelatedWork W1978265572 @default.
• W2022169135 hasRelatedWork W2033112908 @default.
• W2022169135 hasRelatedWork W2036582429 @default.
• W2022169135 hasRelatedWork W2057029395 @default.
• W2022169135 hasRelatedWork W2121253004 @default.

• next