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- W2022170001 abstract "Summary The cap structure from the 5′-ends of the deadenylated protamine mRNAs (PmRNA) was removed with tobacco acid pyrophosphatase. The penultimate 5′-nucleotides were labelled using T4 polynucleotide kinase in the presence of [γ- 32 P]-ATP and the labelled mRNA shown to be intact by electrophoresis on 6% polyacrylamide gels in 8M urea. These “decapped” and labelled PmRNAs do not form 80S initiation complexes and their translation in the wheat germ extract is greatly diminished. This effect may be attributable to the presence of an enzyme in the wheat germ extract with a 5′→3′ exoribonuclease activity which degrades “decapped” PmRNAs, releasing the 5′-terminal 32 P-labelled mononucleotide, 5′-AMP." @default.
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- W2022170001 date "1978-11-01" @default.
- W2022170001 modified "2023-10-14" @default.
- W2022170001 title "Effect of enzymatic “Decapping” on protamine mRNA translation in wheat germ S-30" @default.
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- W2022170001 doi "https://doi.org/10.1016/s0006-291x(78)80018-7" @default.
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