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• W2022172313 abstract "Virulence of Agrobacterium rhizogenes, agent of hairy root disease, is conferred by large plasmids called Ri (root-inducing) plasmids. We have determined the BamHI fragment map of pRi8196, MW 143 Mda, principally by analysis of recombinant plasmids containing overlapping BamHI partial-digest fragments. Clones containing solitary BamHI inserts of remaining unmapped fragments were used to probe a series of Southern-blotted, pRi8196-derived EcoRI, PstI, HindIII, SalI, or SmaI digests. Continguous hybridized bands represented complements of EcoRI, PstI, HindIII, SalI, or SmaI fragments which bridged the unmapped BamHI fragments. We present, in addition, a detailed map of the core T-DNA region with respect to the restriction endonucleases SalI, EcoRI, HpaI, and HindIII." @default.
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• W2022172313 date "1984-01-01" @default.
• W2022172313 modified "2023-09-26" @default.
• W2022172313 title "Physical map of the Agrobacterium rhizogenes strain 8196 virulence plasmid" @default.
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• W2022172313 doi "https://doi.org/10.1016/0147-619x(84)90003-9" @default.
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