Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022179907> ?p ?o ?g. }
- W2022179907 endingPage "2261" @default.
- W2022179907 startingPage "2253" @default.
- W2022179907 abstract "Hybridization analysis of short DNA and RNA targets presents many challenges for detection. The commonly employed sandwich hybridization approach cannot be implemented for these short targets due to insufficient probe-target binding strengths for unmodified DNA probes. Here, we present a method capable of rapid and stable sandwich hybridization detection for 22 nucleotide DNA and RNA targets. Stable hybridization is achieved using an n-alkylated, polyethylene glycol γ-carbon modified peptide nucleic acid (γPNA) amphiphile. The γPNA’s exceptionally high affinity enables stable hybridization of a second DNA-based probe to the remaining bases of the short target. Upon hybridization of both probes, an electrophoretic mobility shift is measured via interaction of the n-alkane modification on the γPNA with capillary electrophoresis running buffer containing nonionic surfactant micelles. We find that sandwich hybridization of both probes is stable under multiple binding configurations and demonstrate single base mismatch discrimination. The binding strength of both probes is also stabilized via coaxial stacking on adjacent hybridization to targets. We conclude with a discussion on the implementation of the proposed sandwich hybridization assay as a high-throughput microRNA detection method." @default.
- W2022179907 created "2016-06-24" @default.
- W2022179907 creator A5028147543 @default.
- W2022179907 creator A5038997100 @default.
- W2022179907 creator A5048920631 @default.
- W2022179907 creator A5060397228 @default.
- W2022179907 creator A5074226700 @default.
- W2022179907 creator A5079664965 @default.
- W2022179907 date "2013-06-18" @default.
- W2022179907 modified "2023-09-23" @default.
- W2022179907 title "High Affinity γPNA Sandwich Hybridization Assay for Rapid Detection of Short Nucleic Acid Targets with Single Mismatch Discrimination" @default.
- W2022179907 cites W1648301895 @default.
- W2022179907 cites W1964878752 @default.
- W2022179907 cites W1969279431 @default.
- W2022179907 cites W1970592466 @default.
- W2022179907 cites W1973582879 @default.
- W2022179907 cites W1976216172 @default.
- W2022179907 cites W1979536696 @default.
- W2022179907 cites W1986948090 @default.
- W2022179907 cites W1993052947 @default.
- W2022179907 cites W2002547315 @default.
- W2022179907 cites W2002995168 @default.
- W2022179907 cites W2005086794 @default.
- W2022179907 cites W2009276329 @default.
- W2022179907 cites W2017392650 @default.
- W2022179907 cites W2017658021 @default.
- W2022179907 cites W2019785555 @default.
- W2022179907 cites W2032129221 @default.
- W2022179907 cites W2044432713 @default.
- W2022179907 cites W2050092543 @default.
- W2022179907 cites W2052278446 @default.
- W2022179907 cites W2053384144 @default.
- W2022179907 cites W2055827668 @default.
- W2022179907 cites W2062752859 @default.
- W2022179907 cites W2064129076 @default.
- W2022179907 cites W2065032657 @default.
- W2022179907 cites W2065814668 @default.
- W2022179907 cites W2072311235 @default.
- W2022179907 cites W2084714412 @default.
- W2022179907 cites W2085296413 @default.
- W2022179907 cites W2091559790 @default.
- W2022179907 cites W2097621920 @default.
- W2022179907 cites W2106045450 @default.
- W2022179907 cites W2112943938 @default.
- W2022179907 cites W2114515553 @default.
- W2022179907 cites W2121096510 @default.
- W2022179907 cites W2126516277 @default.
- W2022179907 cites W2130517519 @default.
- W2022179907 cites W2132383503 @default.
- W2022179907 cites W2132787939 @default.
- W2022179907 cites W2134409042 @default.
- W2022179907 cites W2135995702 @default.
- W2022179907 cites W2136021600 @default.
- W2022179907 cites W2141128504 @default.
- W2022179907 cites W2141728056 @default.
- W2022179907 cites W2141881856 @default.
- W2022179907 cites W2143726250 @default.
- W2022179907 cites W2146241343 @default.
- W2022179907 cites W2158878989 @default.
- W2022179907 cites W2160565454 @default.
- W2022179907 cites W2161222316 @default.
- W2022179907 cites W2166179294 @default.
- W2022179907 cites W2322641183 @default.
- W2022179907 doi "https://doi.org/10.1021/bm400388a" @default.
- W2022179907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23777445" @default.
- W2022179907 hasPublicationYear "2013" @default.
- W2022179907 type Work @default.
- W2022179907 sameAs 2022179907 @default.
- W2022179907 citedByCount "19" @default.
- W2022179907 countsByYear W20221799072013 @default.
- W2022179907 countsByYear W20221799072014 @default.
- W2022179907 countsByYear W20221799072015 @default.
- W2022179907 countsByYear W20221799072016 @default.
- W2022179907 countsByYear W20221799072017 @default.
- W2022179907 countsByYear W20221799072018 @default.
- W2022179907 countsByYear W20221799072019 @default.
- W2022179907 countsByYear W20221799072020 @default.
- W2022179907 countsByYear W20221799072021 @default.
- W2022179907 countsByYear W20221799072022 @default.
- W2022179907 crossrefType "journal-article" @default.
- W2022179907 hasAuthorship W2022179907A5028147543 @default.
- W2022179907 hasAuthorship W2022179907A5038997100 @default.
- W2022179907 hasAuthorship W2022179907A5048920631 @default.
- W2022179907 hasAuthorship W2022179907A5060397228 @default.
- W2022179907 hasAuthorship W2022179907A5074226700 @default.
- W2022179907 hasAuthorship W2022179907A5079664965 @default.
- W2022179907 hasConcept C104317684 @default.
- W2022179907 hasConcept C119203544 @default.
- W2022179907 hasConcept C12554922 @default.
- W2022179907 hasConcept C129312508 @default.
- W2022179907 hasConcept C130197536 @default.
- W2022179907 hasConcept C137390916 @default.
- W2022179907 hasConcept C153911025 @default.
- W2022179907 hasConcept C185592680 @default.
- W2022179907 hasConcept C21951064 @default.
- W2022179907 hasConcept C24107716 @default.
- W2022179907 hasConcept C2777343594 @default.
- W2022179907 hasConcept C54871578 @default.