FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022181520> ?p ?o ?g. }

• W2022181520 endingPage "739" @default.
• W2022181520 startingPage "739" @default.
• W2022181520 abstract "Previously we have demonstrated that relaxin therapy using osmotic minipumps reverses fibrotic cardiomyopathy in rodent models of established fibrosis, including transgenic mice with cardiac-restricted overexpression of β2-adrenergic receptors (β2-TG). In this study, we extended our research by investigating the feasibility of adenoviral-mediated relaxin delivery. Recombinant adenoviruses with a green fluorescent protein (GFP) cassette producing bioactive rat and mouse relaxin peptides (Ad-rRLN, Ad-mRLN, Ad-GFP) were generated. Following amplification and purification the viruses were used to infect HEK-293T cells to characterize their ability to produce bioactive relaxin peptides in vitro. Media from HEK-293T cells infected with Ad-rRLN and Ad-mRLN demonstrated relaxin bioactivity whereas Ad-GFP infected cell media was inactive. The β2-TG mouse model was then used to test efficacy of Ad-mRLN gene therapy. Ad-mRLN together with Ad-GFP as negative control was injected via the tail vein of male and female β2-TG mice at a dose of 5 × 1010pfu. The presence of bioactive relaxin peptide was detected from plasma samples collected at 3-days after injection. Two-weeks post-treatment, the kidneys and hearts were collected and collagen content in the organs was determined by both hydroxyproline assay and quantitative histology. Compared with their wild-type littermates, left ventricular (LV) collagen content was significantly increased in both genders of β2-TG mice (p<0.05 vs non-TG littermates). Adenovirus mediated overexpression of murine relaxin, but not GFP, restored collagen content in the LV of β2-TG to a normal level without adversely affecting collagen levels in other chambers of the heart and organs. Hence, systemic adenoviral relaxin delivery can reverse cardiac fibrosis." @default.
• W2022181520 created "2016-06-24" @default.
• W2022181520 creator A5014646087 @default.
• W2022181520 creator A5029379615 @default.
• W2022181520 creator A5035230144 @default.
• W2022181520 creator A5047695092 @default.
• W2022181520 creator A5057632070 @default.
• W2022181520 creator A5065812198 @default.
• W2022181520 creator A5066565705 @default.
• W2022181520 creator A5081497816 @default.
• W2022181520 creator A5082513643 @default.
• W2022181520 creator A5083070137 @default.
• W2022181520 creator A5088336824 @default.
• W2022181520 date "2006-10-01" @default.
• W2022181520 modified "2023-09-23" @default.
• W2022181520 title "Adenovirus-mediated delivery of relaxin reverses cardiac fibrosis in mice with fibrotic cardiomyopathy" @default.
• W2022181520 doi "https://doi.org/10.1016/j.yjmcc.2006.06.033" @default.
• W2022181520 hasPublicationYear "2006" @default.
• W2022181520 type Work @default.
• W2022181520 sameAs 2022181520 @default.
• W2022181520 citedByCount "1" @default.
• W2022181520 crossrefType "journal-article" @default.
• W2022181520 hasAuthorship W2022181520A5014646087 @default.
• W2022181520 hasAuthorship W2022181520A5029379615 @default.
• W2022181520 hasAuthorship W2022181520A5035230144 @default.
• W2022181520 hasAuthorship W2022181520A5047695092 @default.
• W2022181520 hasAuthorship W2022181520A5057632070 @default.
• W2022181520 hasAuthorship W2022181520A5065812198 @default.
• W2022181520 hasAuthorship W2022181520A5066565705 @default.
• W2022181520 hasAuthorship W2022181520A5081497816 @default.
• W2022181520 hasAuthorship W2022181520A5082513643 @default.
• W2022181520 hasAuthorship W2022181520A5083070137 @default.
• W2022181520 hasAuthorship W2022181520A5088336824 @default.
• W2022181520 hasConcept C102230213 @default.
• W2022181520 hasConcept C104317684 @default.
• W2022181520 hasConcept C111599444 @default.
• W2022181520 hasConcept C126322002 @default.
• W2022181520 hasConcept C134018914 @default.
• W2022181520 hasConcept C135983454 @default.
• W2022181520 hasConcept C142613039 @default.
• W2022181520 hasConcept C153911025 @default.
• W2022181520 hasConcept C170493617 @default.
• W2022181520 hasConcept C174510640 @default.
• W2022181520 hasConcept C185592680 @default.
• W2022181520 hasConcept C202751555 @default.
• W2022181520 hasConcept C2777121736 @default.
• W2022181520 hasConcept C2777420927 @default.
• W2022181520 hasConcept C2778198053 @default.
• W2022181520 hasConcept C2778797674 @default.
• W2022181520 hasConcept C2779797417 @default.
• W2022181520 hasConcept C2780559512 @default.
• W2022181520 hasConcept C40767141 @default.
• W2022181520 hasConcept C54009773 @default.
• W2022181520 hasConcept C54355233 @default.
• W2022181520 hasConcept C55493867 @default.
• W2022181520 hasConcept C71924100 @default.
• W2022181520 hasConcept C81885089 @default.
• W2022181520 hasConcept C86803240 @default.
• W2022181520 hasConceptScore W2022181520C102230213 @default.
• W2022181520 hasConceptScore W2022181520C104317684 @default.
• W2022181520 hasConceptScore W2022181520C111599444 @default.
• W2022181520 hasConceptScore W2022181520C126322002 @default.
• W2022181520 hasConceptScore W2022181520C134018914 @default.
• W2022181520 hasConceptScore W2022181520C135983454 @default.
• W2022181520 hasConceptScore W2022181520C142613039 @default.
• W2022181520 hasConceptScore W2022181520C153911025 @default.
• W2022181520 hasConceptScore W2022181520C170493617 @default.
• W2022181520 hasConceptScore W2022181520C174510640 @default.
• W2022181520 hasConceptScore W2022181520C185592680 @default.
• W2022181520 hasConceptScore W2022181520C202751555 @default.
• W2022181520 hasConceptScore W2022181520C2777121736 @default.
• W2022181520 hasConceptScore W2022181520C2777420927 @default.
• W2022181520 hasConceptScore W2022181520C2778198053 @default.
• W2022181520 hasConceptScore W2022181520C2778797674 @default.
• W2022181520 hasConceptScore W2022181520C2779797417 @default.
• W2022181520 hasConceptScore W2022181520C2780559512 @default.
• W2022181520 hasConceptScore W2022181520C40767141 @default.
• W2022181520 hasConceptScore W2022181520C54009773 @default.
• W2022181520 hasConceptScore W2022181520C54355233 @default.
• W2022181520 hasConceptScore W2022181520C55493867 @default.
• W2022181520 hasConceptScore W2022181520C71924100 @default.
• W2022181520 hasConceptScore W2022181520C81885089 @default.
• W2022181520 hasConceptScore W2022181520C86803240 @default.
• W2022181520 hasIssue "4" @default.
• W2022181520 hasLocation W20221815201 @default.
• W2022181520 hasOpenAccess W2022181520 @default.
• W2022181520 hasPrimaryLocation W20221815201 @default.
• W2022181520 hasRelatedWork W121857568 @default.
• W2022181520 hasRelatedWork W1965530264 @default.
• W2022181520 hasRelatedWork W1968384420 @default.
• W2022181520 hasRelatedWork W2071719941 @default.
• W2022181520 hasRelatedWork W2072345477 @default.
• W2022181520 hasRelatedWork W2249777914 @default.
• W2022181520 hasRelatedWork W2361985732 @default.
• W2022181520 hasRelatedWork W2391890413 @default.
• W2022181520 hasRelatedWork W3143812318 @default.
• W2022181520 hasRelatedWork W770286450 @default.
• W2022181520 hasVolume "41" @default.

• next