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- W2022184112 abstract "Abstract An ultrafiltration technique coupled with liquid chromatography and spectrofluorimetric (λex = 373 nm, λcm = 448 nm) detection is described for rapidly assaying free N-acetylneuraminic acid (Neu5Ac) in the serum of normal and cancer patients. Free Neu5Ac was obtained from serum after ultrafiltration by centrifugation through an Amicon Centrifree micropartition filter which has a relative molecular mass cut-off of 1000 daltons. Aspects of the ultrafiltration procedure include the extent of non-specific binding of Neu5Ac to the ultrafilters, precision of recovery and the effect of temperature. Non-specific losses of Neu5Ac in serum to the ultrafilter system were low (recovery > 91.2%). The detection limit ( S N = 3) = 2 ng/ml . For free Neu5Ac at 180–1421 ng ml−1, the intra- and inter-day C.V.s for the ultrafiltration method were ≤ 2.8% and ≤ 3.9%, respectively. Free Neu5Ac concentrations measured by this method were compared to those measured by the acetonitrile procedure. The results showed an average value of 245 ± 90 ng ml−1 for 30 serum samples of normal subjects. The average value for 20 lung cancer patients was found to be 996 ± 310 ng ml−1." @default.
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- W2022184112 date "1995-04-01" @default.
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- W2022184112 title "Determination of free N-acetyl neuraminic acid by ultrafiltration and liquid chromatography in the serum of normal and cancer patients" @default.
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- W2022184112 doi "https://doi.org/10.1016/0003-2670(94)00577-9" @default.
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