FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022195980> ?p ?o ?g. }

• W2022195980 endingPage "132" @default.
• W2022195980 startingPage "125" @default.
• W2022195980 abstract "Poly(ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA repair, is a target of caspases during apoptosis: its cleavage onto 89- and 24-kDa fragments is considered to be a hallmark of the apoptotic mode of cell death. Another hallmark is the activation of endonuclease which targets internucleosomal DNA. The aim of the present study was to reveal cell cycle phase specificity as well as the temporal and sequence relationships of PARP cleavage vis-à-vis DNA fragmentation in two model systems of apoptosis, one induced by DNA damage via cell treatment with camptothecin (CPT) (mitochondria-induced pathway) and another by the cytotoxic ligand tumor necrosis factor alpha (TNF-alpha) (cell surface death receptor pathway). PARP cleavage was detected immunocytochemically using antibody which recognizes its 89-kDa fragment (PARP p89) while DNA fragmentation was assayed by in situ labeling of DNA strand breaks. The frequency and extent of PARP cleavage as well as DNA fragmentation were measured by mutiparameter flow and laser scanning cytometry. PARP cleavage, selective to S phase cells, was detected 90 min after administration of CPT. PARP cleavage in the cells treated with TNF-alpha was not selective to any cell cycle phase and was seen already after 30 min. DNA fragmentation trailed PARP cleavage by about 30 min and showed a similar pattern of cell cycle specificity. PARP p89 was present in nuclear chromatin but at least in the early phase of apoptosis it did not colocalize with DNA strand breaks. The rate of cleavage of PARP molecules in individual cells whether induced by CPT or TNF-alpha was rapid as reflected by the paucity of cells with a mixture of cleaved and noncleaved PARP molecules. In contrast, DNA fragmentation proceeded stepwise before reaching the maximal number of DNA strand breaks. Although time windows for PARP cleavage vs DNA fragmentation were different at early stages of apoptosis, a good overall correlation between the cytometric assays of apoptotic cells identification based on these events was observed in both CPT- and TNF-alpha-treated cultures." @default.
• W2022195980 created "2016-06-24" @default.
• W2022195980 creator A5015640756 @default.
• W2022195980 creator A5033158298 @default.
• W2022195980 date "2000-02-01" @default.
• W2022195980 modified "2023-10-16" @default.
• W2022195980 title "Cleavage of Poly(ADP-Ribose) Polymerase Measured in Situ in Individual Cells: Relationship to DNA Fragmentation and Cell Cycle Position during Apoptosis" @default.
• W2022195980 cites W1967912613 @default.
• W2022195980 cites W1980880143 @default.
• W2022195980 cites W1982577514 @default.
• W2022195980 cites W1991253583 @default.
• W2022195980 cites W1998875539 @default.
• W2022195980 cites W2001866311 @default.
• W2022195980 cites W2002503549 @default.
• W2022195980 cites W2008032729 @default.
• W2022195980 cites W2008901624 @default.
• W2022195980 cites W2010298858 @default.
• W2022195980 cites W2014300705 @default.
• W2022195980 cites W2036822121 @default.
• W2022195980 cites W2036937264 @default.
• W2022195980 cites W2040955353 @default.
• W2022195980 cites W2060048383 @default.
• W2022195980 cites W2068147094 @default.
• W2022195980 cites W2068790232 @default.
• W2022195980 cites W2073433611 @default.
• W2022195980 cites W2093715312 @default.
• W2022195980 cites W2117743516 @default.
• W2022195980 cites W2127429864 @default.
• W2022195980 cites W2151501496 @default.
• W2022195980 cites W2154341587 @default.
• W2022195980 cites W2165436746 @default.
• W2022195980 cites W2168378904 @default.
• W2022195980 cites W4241419378 @default.
• W2022195980 cites W4245361947 @default.
• W2022195980 cites W4251350045 @default.
• W2022195980 cites W4252921418 @default.
• W2022195980 doi "https://doi.org/10.1006/excr.1999.4796" @default.
• W2022195980 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10666341" @default.
• W2022195980 hasPublicationYear "2000" @default.
• W2022195980 type Work @default.
• W2022195980 sameAs 2022195980 @default.
• W2022195980 citedByCount "112" @default.
• W2022195980 countsByYear W20221959802012 @default.
• W2022195980 countsByYear W20221959802013 @default.
• W2022195980 countsByYear W20221959802014 @default.
• W2022195980 countsByYear W20221959802015 @default.
• W2022195980 countsByYear W20221959802016 @default.
• W2022195980 countsByYear W20221959802017 @default.
• W2022195980 countsByYear W20221959802018 @default.
• W2022195980 countsByYear W20221959802020 @default.
• W2022195980 countsByYear W20221959802021 @default.
• W2022195980 countsByYear W20221959802022 @default.
• W2022195980 countsByYear W20221959802023 @default.
• W2022195980 crossrefType "journal-article" @default.
• W2022195980 hasAuthorship W2022195980A5015640756 @default.
• W2022195980 hasAuthorship W2022195980A5033158298 @default.
• W2022195980 hasConcept C143425029 @default.
• W2022195980 hasConcept C151730666 @default.
• W2022195980 hasConcept C153911025 @default.
• W2022195980 hasConcept C175156509 @default.
• W2022195980 hasConcept C182979987 @default.
• W2022195980 hasConcept C18903297 @default.
• W2022195980 hasConcept C190283241 @default.
• W2022195980 hasConcept C191015642 @default.
• W2022195980 hasConcept C29537977 @default.
• W2022195980 hasConcept C31573885 @default.
• W2022195980 hasConcept C43369102 @default.
• W2022195980 hasConcept C43759708 @default.
• W2022195980 hasConcept C552990157 @default.
• W2022195980 hasConcept C55493867 @default.
• W2022195980 hasConcept C82381507 @default.
• W2022195980 hasConcept C86803240 @default.
• W2022195980 hasConcept C87439868 @default.
• W2022195980 hasConcept C95444343 @default.
• W2022195980 hasConceptScore W2022195980C143425029 @default.
• W2022195980 hasConceptScore W2022195980C151730666 @default.
• W2022195980 hasConceptScore W2022195980C153911025 @default.
• W2022195980 hasConceptScore W2022195980C175156509 @default.
• W2022195980 hasConceptScore W2022195980C182979987 @default.
• W2022195980 hasConceptScore W2022195980C18903297 @default.
• W2022195980 hasConceptScore W2022195980C190283241 @default.
• W2022195980 hasConceptScore W2022195980C191015642 @default.
• W2022195980 hasConceptScore W2022195980C29537977 @default.
• W2022195980 hasConceptScore W2022195980C31573885 @default.
• W2022195980 hasConceptScore W2022195980C43369102 @default.
• W2022195980 hasConceptScore W2022195980C43759708 @default.
• W2022195980 hasConceptScore W2022195980C552990157 @default.
• W2022195980 hasConceptScore W2022195980C55493867 @default.
• W2022195980 hasConceptScore W2022195980C82381507 @default.
• W2022195980 hasConceptScore W2022195980C86803240 @default.
• W2022195980 hasConceptScore W2022195980C87439868 @default.
• W2022195980 hasConceptScore W2022195980C95444343 @default.
• W2022195980 hasIssue "1" @default.
• W2022195980 hasLocation W20221959801 @default.
• W2022195980 hasLocation W20221959802 @default.
• W2022195980 hasOpenAccess W2022195980 @default.
• W2022195980 hasPrimaryLocation W20221959801 @default.
• W2022195980 hasRelatedWork W1432933144 @default.

• next