FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022197835> ?p ?o ?g. }

• W2022197835 endingPage "489" @default.
• W2022197835 startingPage "479" @default.
• W2022197835 abstract "The key event in the switch from lysogenic to lytic growth of phage lambda is the self-cleavage of lambda repressor, which is induced by the formation of a RecA-ssDNA-ATP filament at a site of DNA damage. Lambda repressor cleaves itself at the peptide bond between Ala111 and Gly112, but only when bound as a monomer to the RecA-ssDNA-ATP filament. Here we have designed a hyper-cleavable fragment of lambda repressor containing the hinge and C-terminal domain (residues 101–229), in which the monomer–monomer interface is disrupted by two point mutations and a deletion of seven residues at the C terminus. This fragment crystallizes as a monomer and its structure has been determined to 1.8 Å resolution. The hinge region, which bears the cleavage site, is folded over the active site of the C-terminal oligomerization domain (CTD) but with the cleavage site flipped out and exposed to solvent. Thus, the structure represents a non-cleavable conformation of the repressor, but one that is poised for cleavage after modest rearrangements that are presumably stabilized by binding to RecA. The structure provides a unique snapshot of lambda repressor in a conformation that sheds light on how its self-cleavage is tempered in the absence of RecA, as well as a framework for interpreting previous genetic and biochemical data concerning the RecA-mediated cleavage reaction." @default.
• W2022197835 created "2016-06-24" @default.
• W2022197835 creator A5067691635 @default.
• W2022197835 creator A5074019511 @default.
• W2022197835 date "2006-09-01" @default.
• W2022197835 modified "2023-09-27" @default.
• W2022197835 title "Structure of a Hyper-cleavable Monomeric Fragment of Phage λ Repressor Containing the Cleavage Site Region" @default.
• W2022197835 cites W1546072668 @default.
• W2022197835 cites W1546865473 @default.
• W2022197835 cites W1604437810 @default.
• W2022197835 cites W1979260009 @default.
• W2022197835 cites W1980452218 @default.
• W2022197835 cites W1991998153 @default.
• W2022197835 cites W1992210744 @default.
• W2022197835 cites W1995017064 @default.
• W2022197835 cites W1995080182 @default.
• W2022197835 cites W2002848104 @default.
• W2022197835 cites W2002866511 @default.
• W2022197835 cites W2009912933 @default.
• W2022197835 cites W2013083986 @default.
• W2022197835 cites W2015706250 @default.
• W2022197835 cites W2016281336 @default.
• W2022197835 cites W2025351606 @default.
• W2022197835 cites W2026598193 @default.
• W2022197835 cites W2027087381 @default.
• W2022197835 cites W2029798964 @default.
• W2022197835 cites W2042578191 @default.
• W2022197835 cites W2044841023 @default.
• W2022197835 cites W2049279850 @default.
• W2022197835 cites W2068572804 @default.
• W2022197835 cites W2070538799 @default.
• W2022197835 cites W2074586722 @default.
• W2022197835 cites W2080973820 @default.
• W2022197835 cites W2089224334 @default.
• W2022197835 cites W2113495126 @default.
• W2022197835 doi "https://doi.org/10.1016/j.jmb.2006.07.026" @default.
• W2022197835 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1896146" @default.
• W2022197835 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16934834" @default.
• W2022197835 hasPublicationYear "2006" @default.
• W2022197835 type Work @default.
• W2022197835 sameAs 2022197835 @default.
• W2022197835 citedByCount "18" @default.
• W2022197835 countsByYear W20221978352013 @default.
• W2022197835 countsByYear W20221978352018 @default.
• W2022197835 countsByYear W20221978352019 @default.
• W2022197835 countsByYear W20221978352020 @default.
• W2022197835 countsByYear W20221978352022 @default.
• W2022197835 countsByYear W20221978352023 @default.
• W2022197835 crossrefType "journal-article" @default.
• W2022197835 hasAuthorship W2022197835A5067691635 @default.
• W2022197835 hasAuthorship W2022197835A5074019511 @default.
• W2022197835 hasBestOaLocation W20221978352 @default.
• W2022197835 hasConcept C104317684 @default.
• W2022197835 hasConcept C107824862 @default.
• W2022197835 hasConcept C133672593 @default.
• W2022197835 hasConcept C136199206 @default.
• W2022197835 hasConcept C151730666 @default.
• W2022197835 hasConcept C158448853 @default.
• W2022197835 hasConcept C166940927 @default.
• W2022197835 hasConcept C175156509 @default.
• W2022197835 hasConcept C178790620 @default.
• W2022197835 hasConcept C182615771 @default.
• W2022197835 hasConcept C185592680 @default.
• W2022197835 hasConcept C2522874641 @default.
• W2022197835 hasConcept C2776441376 @default.
• W2022197835 hasConcept C2779546866 @default.
• W2022197835 hasConcept C2781135666 @default.
• W2022197835 hasConcept C43369102 @default.
• W2022197835 hasConcept C515207424 @default.
• W2022197835 hasConcept C521977710 @default.
• W2022197835 hasConcept C54355233 @default.
• W2022197835 hasConcept C547475151 @default.
• W2022197835 hasConcept C552990157 @default.
• W2022197835 hasConcept C55493867 @default.
• W2022197835 hasConcept C57711820 @default.
• W2022197835 hasConcept C67705224 @default.
• W2022197835 hasConcept C71240020 @default.
• W2022197835 hasConcept C86339819 @default.
• W2022197835 hasConcept C86803240 @default.
• W2022197835 hasConceptScore W2022197835C104317684 @default.
• W2022197835 hasConceptScore W2022197835C107824862 @default.
• W2022197835 hasConceptScore W2022197835C133672593 @default.
• W2022197835 hasConceptScore W2022197835C136199206 @default.
• W2022197835 hasConceptScore W2022197835C151730666 @default.
• W2022197835 hasConceptScore W2022197835C158448853 @default.
• W2022197835 hasConceptScore W2022197835C166940927 @default.
• W2022197835 hasConceptScore W2022197835C175156509 @default.
• W2022197835 hasConceptScore W2022197835C178790620 @default.
• W2022197835 hasConceptScore W2022197835C182615771 @default.
• W2022197835 hasConceptScore W2022197835C185592680 @default.
• W2022197835 hasConceptScore W2022197835C2522874641 @default.
• W2022197835 hasConceptScore W2022197835C2776441376 @default.
• W2022197835 hasConceptScore W2022197835C2779546866 @default.
• W2022197835 hasConceptScore W2022197835C2781135666 @default.
• W2022197835 hasConceptScore W2022197835C43369102 @default.
• W2022197835 hasConceptScore W2022197835C515207424 @default.
• W2022197835 hasConceptScore W2022197835C521977710 @default.
• W2022197835 hasConceptScore W2022197835C54355233 @default.

• next