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• W2022211106 abstract "Dental pulp destruction is believed to be regulated, in part, by the matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs). Cytokines are believed to be important in the pathogenesis of pulpitis. This study examined the effects that TNF-alpha, IL-1beta, IL-6, and TGF-beta1 have on the collagen degradation mediated by pulp fibroblasts utilizing a cell-mediated collagen degradation assay. Reverse transcriptase-polymerase chain reaction, Western blot analyses, and zymography were utilized to examine multiple MMPs and TIMPs. The collagen degradation mediated by these cells was stimulated by these cytokines. TNF-alpha, IL-1beta, and IL-6 increased the mRNA and/or protein expression of MMP-1, MMP-2, and MMP-3. TGF-beta1 decreased MMP-1 mRNA expression, while only slightly affecting the MMP-2 and MMP-3 mRNA and/or protein. These cytokines did not affect the expression of TIMP-1 or TIMP-2. These results suggest that these cytokines affect pulp destruction, in part, by differentially regulating the MMPs and TIMPs." @default.
• W2022211106 created "2016-06-24" @default.
• W2022211106 creator A5015118901 @default.
• W2022211106 creator A5049507979 @default.
• W2022211106 date "2006-09-01" @default.
• W2022211106 modified "2023-10-01" @default.
• W2022211106 title "The Effects of Tumor Necrosis Factor-α, Interleukin-1β, Interleukin-6, and Transforming Growth Factor-β1 on Pulp Fibroblast Mediated Collagen Degradation" @default.
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• W2022211106 doi "https://doi.org/10.1016/j.joen.2006.03.017" @default.
• W2022211106 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16934628" @default.
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