FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022219000> ?p ?o ?g. }

• W2022219000 endingPage "265" @default.
• W2022219000 startingPage "253" @default.
• W2022219000 abstract "1. The mechanism underlying the vasoconstriction induced by endothelin-1 (ET-1) was investigated by measuring the intracellular concentration of Ca2+ ([Ca2+]i), isometric force and phosphorylation of the myosin light chain (MLC) in endothelium-denuded unskinned and beta-escin-treated skinned smooth muscle from resistance vessels of the rabbit mesentery. The role of protein kinase C (PKC) in the action of ET-1 was studied in skinned smooth muscle using a synthetic peptide, PKC19-36, which corresponds to the autoinhibitory domain of PKC. 2. ET-1 (> 0.1 nM) induced slowly developing, maintained increases in [Ca2+]i and force. Nicardipine completely blocked the ET-1-induced increase in [Ca2+]i. BQ-123 (an inhibitor of the ETA receptor) blocked the ET-1-induced contraction but IRL 1620 (Suc-[Glu9,Ala11,15]-ET-1(8-21), an agonist of the ETB receptor) failed to induce contraction. 3. In ionomycin- and 70 mM K(+)-treated strips, ET-1 shifted the [Ca2+]i-force relationship to the left and enhanced the maximum amplitude of contraction induced by 2.6 mM Ca2+. In skinned smooth muscle treated with ionomycin, Ca2+ (0.1-3 microM) increased both force and MLC phosphorylation, in a concentration-dependent manner. ET-1 with GTP shifted both the Ca(2+)-force and Ca(2+)-MLC phosphorylation relationships to the left without significant changes in the maximum responses. ET-1 with GTP did not change the relationship between force and MLC phosphorylation. Similar effects were observed with phorbol 12,13-dibutyrate (PDBu, an activator of PKC). These results indicate that the sensitivity of MLC phosphorylation to Ca2+ is enhanced both by ET-1 with GTP and by PDBu. 4. PKC19-36 (an inhibitor of PKC) modified neither the contraction nor MLC phosphorylation induced by 0.3 microM Ca2+ but blocked the PDBu-induced enhancement of both these Ca(2+)-induced responses. However, PKC19-36 only partly inhibited the enhancement produced by ET-1 with GTP on the Ca(2+)-induced responses. PKC19-36 did not modify the relationship between force and MLC phosphorylation in the presence either of ET-1 with GTP or of PDBu. By contrast, BQ-123, neomycin and guanosine 5'-O-(2-thiodiphosphate) (GDP beta S) each abolished the ET-1-induced enhancement of the contraction induced by 0.3 microM Ca2+. 5. These results suggest that ET-1 acts on the ETA receptor and increases Ca2+ influxes through an activation of the dihydropyridine-sensitive Ca2+ channel, causing a long-lasting and maintained contraction in resistance vessels of the rabbit mesentery.(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
• W2022219000 created "2016-06-24" @default.
• W2022219000 creator A5018665174 @default.
• W2022219000 creator A5020037667 @default.
• W2022219000 creator A5022447092 @default.
• W2022219000 date "1994-06-01" @default.
• W2022219000 modified "2023-09-24" @default.
• W2022219000 title "Mechanisms of vasoconstriction induced by endothelin-1 in smooth muscle of rabbit mesenteric artery." @default.
• W2022219000 cites W1507228972 @default.
• W2022219000 cites W1514066364 @default.
• W2022219000 cites W1522733018 @default.
• W2022219000 cites W1542006519 @default.
• W2022219000 cites W1564154324 @default.
• W2022219000 cites W1577867292 @default.
• W2022219000 cites W1596968588 @default.
• W2022219000 cites W1597139514 @default.
• W2022219000 cites W1674539446 @default.
• W2022219000 cites W1972262110 @default.
• W2022219000 cites W1973454785 @default.
• W2022219000 cites W1983184640 @default.
• W2022219000 cites W1990700070 @default.
• W2022219000 cites W1996024251 @default.
• W2022219000 cites W2000884517 @default.
• W2022219000 cites W2003268498 @default.
• W2022219000 cites W2004917770 @default.
• W2022219000 cites W2005189737 @default.
• W2022219000 cites W2018653588 @default.
• W2022219000 cites W2046911701 @default.
• W2022219000 cites W2050338578 @default.
• W2022219000 cites W2053502416 @default.
• W2022219000 cites W2059369537 @default.
• W2022219000 cites W2060117625 @default.
• W2022219000 cites W2078660519 @default.
• W2022219000 cites W2084935848 @default.
• W2022219000 cites W2091673674 @default.
• W2022219000 cites W2093113620 @default.
• W2022219000 cites W2093655696 @default.
• W2022219000 cites W2126449762 @default.
• W2022219000 cites W2141260882 @default.
• W2022219000 cites W2151869895 @default.
• W2022219000 cites W2162804111 @default.
• W2022219000 cites W2253687991 @default.
• W2022219000 cites W2411195788 @default.
• W2022219000 cites W2417997589 @default.
• W2022219000 cites W52268341 @default.
• W2022219000 doi "https://doi.org/10.1113/jphysiol.1994.sp020188" @default.
• W2022219000 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1155626" @default.
• W2022219000 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7932217" @default.
• W2022219000 hasPublicationYear "1994" @default.
• W2022219000 type Work @default.
• W2022219000 sameAs 2022219000 @default.
• W2022219000 citedByCount "59" @default.
• W2022219000 countsByYear W20222190002012 @default.
• W2022219000 countsByYear W20222190002014 @default.
• W2022219000 countsByYear W20222190002015 @default.
• W2022219000 countsByYear W20222190002016 @default.
• W2022219000 countsByYear W20222190002017 @default.
• W2022219000 countsByYear W20222190002019 @default.
• W2022219000 countsByYear W20222190002020 @default.
• W2022219000 countsByYear W20222190002023 @default.
• W2022219000 crossrefType "journal-article" @default.
• W2022219000 hasAuthorship W2022219000A5018665174 @default.
• W2022219000 hasAuthorship W2022219000A5020037667 @default.
• W2022219000 hasAuthorship W2022219000A5022447092 @default.
• W2022219000 hasBestOaLocation W20222190002 @default.
• W2022219000 hasConcept C11960822 @default.
• W2022219000 hasConcept C12554922 @default.
• W2022219000 hasConcept C126322002 @default.
• W2022219000 hasConcept C134018914 @default.
• W2022219000 hasConcept C144980905 @default.
• W2022219000 hasConcept C149601957 @default.
• W2022219000 hasConcept C163415756 @default.
• W2022219000 hasConcept C170493617 @default.
• W2022219000 hasConcept C185592680 @default.
• W2022219000 hasConcept C195794163 @default.
• W2022219000 hasConcept C2776989580 @default.
• W2022219000 hasConcept C2779961880 @default.
• W2022219000 hasConcept C2909649922 @default.
• W2022219000 hasConcept C519063684 @default.
• W2022219000 hasConcept C55493867 @default.
• W2022219000 hasConcept C71924100 @default.
• W2022219000 hasConcept C8035138 @default.
• W2022219000 hasConcept C86803240 @default.
• W2022219000 hasConceptScore W2022219000C11960822 @default.
• W2022219000 hasConceptScore W2022219000C12554922 @default.
• W2022219000 hasConceptScore W2022219000C126322002 @default.
• W2022219000 hasConceptScore W2022219000C134018914 @default.
• W2022219000 hasConceptScore W2022219000C144980905 @default.
• W2022219000 hasConceptScore W2022219000C149601957 @default.
• W2022219000 hasConceptScore W2022219000C163415756 @default.
• W2022219000 hasConceptScore W2022219000C170493617 @default.
• W2022219000 hasConceptScore W2022219000C185592680 @default.
• W2022219000 hasConceptScore W2022219000C195794163 @default.
• W2022219000 hasConceptScore W2022219000C2776989580 @default.
• W2022219000 hasConceptScore W2022219000C2779961880 @default.
• W2022219000 hasConceptScore W2022219000C2909649922 @default.
• W2022219000 hasConceptScore W2022219000C519063684 @default.
• W2022219000 hasConceptScore W2022219000C55493867 @default.

• next