FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022225492> ?p ?o ?g. }

• W2022225492 endingPage "1444" @default.
• W2022225492 startingPage "1434" @default.
• W2022225492 abstract "Transgenic plants are potentially valuable systems for the large scale manufacture of therapeutic proteins. To improve this technology, determining the importance of transgene transcript levels on protein accumulation in sink tissues during their development is crucial. In transgenic maize (Zea mays L.) plants expressing humanized monoclonal antibodies (mAbs) in their seed endosperm, steady-state κ light chain (LC) and γ heavy chain (HC) mRNA levels were quantified during development and compared to the levels of fully-assembled mAb protein present at seed maturity. RNA blots and non-reducing SDS-PAGE western immunoblots revealed that steady-state LC and HC mRNA and protein levels were undetectable at 10 days after pollination (DAP) but increased quickly thereafter in three transgenic events expressing different mAb molecules. Similar to γ-zein mRNA, LC and HC messages were highly abundant between 15 and 25 DAP. Quantitative RNA blots and western immunoblots showed that steady-state LC transcript levels during development correlated extremely closely with protein levels in mature seed (r2 = 0.99). For HC, this correlation was not as strong (r2 = 0.85). Consistent with this finding, concomitantly increasing the zygosity levels of the LC and HC transgenes enhanced mAb concentration in mature seed, in contrast to increasing the copy number of the transgene insert, which did not correlate with high seed mAb levels. The results indicate that high-level expression of fully-assembled mAb protein in maize endosperm was favored by high LC and HC mRNA levels and was largely limited by HC protein concentration." @default.
• W2022225492 created "2016-06-24" @default.
• W2022225492 creator A5002055138 @default.
• W2022225492 creator A5005520053 @default.
• W2022225492 creator A5030809311 @default.
• W2022225492 creator A5032024840 @default.
• W2022225492 creator A5035386203 @default.
• W2022225492 creator A5053395032 @default.
• W2022225492 creator A5065802314 @default.
• W2022225492 creator A5072117959 @default.
• W2022225492 creator A5090484655 @default.
• W2022225492 date "2006-09-01" @default.
• W2022225492 modified "2023-09-25" @default.
• W2022225492 title "Biochemical limitations to high-level expression of humanized monoclonal antibodies in transgenic maize seed endosperm" @default.
• W2022225492 cites W1217209530 @default.
• W2022225492 cites W1278420624 @default.
• W2022225492 cites W1498806091 @default.
• W2022225492 cites W1502786433 @default.
• W2022225492 cites W1581043340 @default.
• W2022225492 cites W1583192328 @default.
• W2022225492 cites W1588334794 @default.
• W2022225492 cites W1771101846 @default.
• W2022225492 cites W1966491512 @default.
• W2022225492 cites W1976650652 @default.
• W2022225492 cites W1984826216 @default.
• W2022225492 cites W1986474909 @default.
• W2022225492 cites W1990313556 @default.
• W2022225492 cites W1994017824 @default.
• W2022225492 cites W1995562003 @default.
• W2022225492 cites W1996512321 @default.
• W2022225492 cites W1997837553 @default.
• W2022225492 cites W1999643340 @default.
• W2022225492 cites W2006146698 @default.
• W2022225492 cites W20153280 @default.
• W2022225492 cites W2024266618 @default.
• W2022225492 cites W2026777911 @default.
• W2022225492 cites W2027081061 @default.
• W2022225492 cites W2029114240 @default.
• W2022225492 cites W2042455517 @default.
• W2022225492 cites W2051157535 @default.
• W2022225492 cites W2060204419 @default.
• W2022225492 cites W2073754603 @default.
• W2022225492 cites W2076047492 @default.
• W2022225492 cites W2077784911 @default.
• W2022225492 cites W2078716988 @default.
• W2022225492 cites W2081575769 @default.
• W2022225492 cites W2086270091 @default.
• W2022225492 cites W2091785743 @default.
• W2022225492 cites W2097043803 @default.
• W2022225492 cites W2101538287 @default.
• W2022225492 cites W2110311890 @default.
• W2022225492 cites W2123018879 @default.
• W2022225492 cites W2158714788 @default.
• W2022225492 cites W2167283605 @default.
• W2022225492 cites W2191356210 @default.
• W2022225492 cites W236382064 @default.
• W2022225492 cites W4231639351 @default.
• W2022225492 cites W83210865 @default.
• W2022225492 cites W87063270 @default.
• W2022225492 doi "https://doi.org/10.1016/j.bbagen.2006.05.006" @default.
• W2022225492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16842925" @default.
• W2022225492 hasPublicationYear "2006" @default.
• W2022225492 type Work @default.
• W2022225492 sameAs 2022225492 @default.
• W2022225492 citedByCount "18" @default.
• W2022225492 countsByYear W20222254922012 @default.
• W2022225492 countsByYear W20222254922014 @default.
• W2022225492 countsByYear W20222254922015 @default.
• W2022225492 countsByYear W20222254922016 @default.
• W2022225492 countsByYear W20222254922017 @default.
• W2022225492 countsByYear W20222254922019 @default.
• W2022225492 countsByYear W20222254922021 @default.
• W2022225492 countsByYear W20222254922022 @default.
• W2022225492 crossrefType "journal-article" @default.
• W2022225492 hasAuthorship W2022225492A5002055138 @default.
• W2022225492 hasAuthorship W2022225492A5005520053 @default.
• W2022225492 hasAuthorship W2022225492A5030809311 @default.
• W2022225492 hasAuthorship W2022225492A5032024840 @default.
• W2022225492 hasAuthorship W2022225492A5035386203 @default.
• W2022225492 hasAuthorship W2022225492A5053395032 @default.
• W2022225492 hasAuthorship W2022225492A5065802314 @default.
• W2022225492 hasAuthorship W2022225492A5072117959 @default.
• W2022225492 hasAuthorship W2022225492A5090484655 @default.
• W2022225492 hasConcept C102230213 @default.
• W2022225492 hasConcept C104317684 @default.
• W2022225492 hasConcept C105580179 @default.
• W2022225492 hasConcept C113442657 @default.
• W2022225492 hasConcept C150194340 @default.
• W2022225492 hasConcept C153911025 @default.
• W2022225492 hasConcept C159654299 @default.
• W2022225492 hasConcept C2776415932 @default.
• W2022225492 hasConcept C542903549 @default.
• W2022225492 hasConcept C54355233 @default.
• W2022225492 hasConcept C55493867 @default.
• W2022225492 hasConcept C85265743 @default.
• W2022225492 hasConcept C86803240 @default.
• W2022225492 hasConceptScore W2022225492C102230213 @default.
• W2022225492 hasConceptScore W2022225492C104317684 @default.

• next