Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022226051> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2022226051 endingPage "0" @default.
- W2022226051 startingPage "0" @default.
- W2022226051 abstract "Purpose Amyloid beta, the main protein component of Alzheimer (AD) plaques and tangles, is characterized by high levels of beta-sheets. Raman microspectroscopy allows quantitative analysis of this specific molecular conformation. We compared the beta-sheet levels in lens opacities and in plaques and tangles in the hippocampus of AD patients. Methods We obtained 14 lenses from 7 post-mortem donors , neuropathologically confirmed as having advanced or moderate AD. From 3 of these donors, we also obtained hippocampus tissue. Protein and lipid conformations were analysed in the 500-1800 cm-1 fingerprint region. The ratio of the beta-sheet peak at 1668 cm-1 and the protein peak at 1450 cm-1 is a quantitative measure for the beta-sheet content. Additionally, histological sections were stained using a standard Congo red protocol and amyloid beta immunohistochemistry. Results The 1668cm-1/1450cm-1 ratio, quantitatively reflecting the beta-sheet content, is 1.28 for clear and cataractous regions in the lens.For the plaques and tangles in the hippocampus the ratio is 1.62 and 1.23 for non-affected regions. When corrected for the presence of lipids in plaques and tangles this ratio is 2.64. Congo red and amyloid beta immunohistochemistry is positive for AD plaques and tangles but is negative for all lenses studied. Conclusion In contrast with a previous study (Goldstein et al. 2003) we conclude that proteins in opaque regions of lenses and in hippocampal plaques and tangles are fully different species. Moreover opacification is not accompanied by changes in the beta-sheet configuration of lens proteins. This means that cortical cataract cannot be considered as an indicator and predictor of AD." @default.
- W2022226051 created "2016-06-24" @default.
- W2022226051 creator A5015264370 @default.
- W2022226051 creator A5020057579 @default.
- W2022226051 creator A5047812313 @default.
- W2022226051 creator A5048259756 @default.
- W2022226051 creator A5052985355 @default.
- W2022226051 creator A5062865460 @default.
- W2022226051 creator A5074792748 @default.
- W2022226051 date "2013-08-01" @default.
- W2022226051 modified "2023-09-27" @default.
- W2022226051 title "Absence of amyloid beta in human lens opacities: A confocal Raman study" @default.
- W2022226051 doi "https://doi.org/10.1111/j.1755-3768.2013.s079.x" @default.
- W2022226051 hasPublicationYear "2013" @default.
- W2022226051 type Work @default.
- W2022226051 sameAs 2022226051 @default.
- W2022226051 citedByCount "0" @default.
- W2022226051 crossrefType "journal-article" @default.
- W2022226051 hasAuthorship W2022226051A5015264370 @default.
- W2022226051 hasAuthorship W2022226051A5020057579 @default.
- W2022226051 hasAuthorship W2022226051A5047812313 @default.
- W2022226051 hasAuthorship W2022226051A5048259756 @default.
- W2022226051 hasAuthorship W2022226051A5052985355 @default.
- W2022226051 hasAuthorship W2022226051A5062865460 @default.
- W2022226051 hasAuthorship W2022226051A5074792748 @default.
- W2022226051 hasBestOaLocation W20222260511 @default.
- W2022226051 hasConcept C120665830 @default.
- W2022226051 hasConcept C121332964 @default.
- W2022226051 hasConcept C126322002 @default.
- W2022226051 hasConcept C142724271 @default.
- W2022226051 hasConcept C150394285 @default.
- W2022226051 hasConcept C178790620 @default.
- W2022226051 hasConcept C185592680 @default.
- W2022226051 hasConcept C199360897 @default.
- W2022226051 hasConcept C204232928 @default.
- W2022226051 hasConcept C2776174256 @default.
- W2022226051 hasConcept C2777633098 @default.
- W2022226051 hasConcept C2779134260 @default.
- W2022226051 hasConcept C2779155187 @default.
- W2022226051 hasConcept C2781161787 @default.
- W2022226051 hasConcept C40003534 @default.
- W2022226051 hasConcept C41008148 @default.
- W2022226051 hasConcept C502032728 @default.
- W2022226051 hasConcept C68026918 @default.
- W2022226051 hasConcept C71924100 @default.
- W2022226051 hasConceptScore W2022226051C120665830 @default.
- W2022226051 hasConceptScore W2022226051C121332964 @default.
- W2022226051 hasConceptScore W2022226051C126322002 @default.
- W2022226051 hasConceptScore W2022226051C142724271 @default.
- W2022226051 hasConceptScore W2022226051C150394285 @default.
- W2022226051 hasConceptScore W2022226051C178790620 @default.
- W2022226051 hasConceptScore W2022226051C185592680 @default.
- W2022226051 hasConceptScore W2022226051C199360897 @default.
- W2022226051 hasConceptScore W2022226051C204232928 @default.
- W2022226051 hasConceptScore W2022226051C2776174256 @default.
- W2022226051 hasConceptScore W2022226051C2777633098 @default.
- W2022226051 hasConceptScore W2022226051C2779134260 @default.
- W2022226051 hasConceptScore W2022226051C2779155187 @default.
- W2022226051 hasConceptScore W2022226051C2781161787 @default.
- W2022226051 hasConceptScore W2022226051C40003534 @default.
- W2022226051 hasConceptScore W2022226051C41008148 @default.
- W2022226051 hasConceptScore W2022226051C502032728 @default.
- W2022226051 hasConceptScore W2022226051C68026918 @default.
- W2022226051 hasConceptScore W2022226051C71924100 @default.
- W2022226051 hasLocation W20222260511 @default.
- W2022226051 hasOpenAccess W2022226051 @default.
- W2022226051 hasPrimaryLocation W20222260511 @default.
- W2022226051 hasRelatedWork W1532336307 @default.
- W2022226051 hasRelatedWork W1980649004 @default.
- W2022226051 hasRelatedWork W2002275590 @default.
- W2022226051 hasRelatedWork W2013775559 @default.
- W2022226051 hasRelatedWork W2029547769 @default.
- W2022226051 hasRelatedWork W2030100345 @default.
- W2022226051 hasRelatedWork W2055177082 @default.
- W2022226051 hasRelatedWork W2076272666 @default.
- W2022226051 hasRelatedWork W2130393399 @default.
- W2022226051 hasRelatedWork W2280733710 @default.
- W2022226051 hasVolume "91" @default.
- W2022226051 isParatext "false" @default.
- W2022226051 isRetracted "false" @default.
- W2022226051 magId "2022226051" @default.
- W2022226051 workType "article" @default.