Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022227305> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2022227305 endingPage "591" @default.
- W2022227305 startingPage "585" @default.
- W2022227305 abstract "Background: Whole genome amplification (WGA) is usually needed in the genetic analysis of samples containing a low number of cells. In genome-wide analysis of DNA copy numbers by array comparative genomic hybridization (array-CGH) it is very important that the genome is evenly represented throughout the amplified product. All currently available WGA techniques are generating some degree of bias. Methods: A way to compensate for this is using a reference sample which is similarly amplified, as the introduced amplification bias will be leveled out. Additionally, direct labeling of the amplified DNA is performed to bypass the currently widely applied random primed labeling, which involves an additional amplification of the product and is introducing extra bias. Results: In this article it is shown that equal processing of the test and reference sample is indeed crucial to generate an optimal array-CGH profile of amplified DNA samples. Also presented here is that the labeling method may significantly effect the array-CGH result, it is shown that with direct chemical labeling using platinum derivates (ULS labeling) optimal array-CGH results are obtained. Conclusions: We show that an optimized WGA strategy for both test and reference sample in combination with direct chemical labeling results in a reliable array-CGH profile of samples as low as a 30 cell equivalent. © 2007 International Society for Analytical Cytology" @default.
- W2022227305 created "2016-06-24" @default.
- W2022227305 creator A5004404120 @default.
- W2022227305 creator A5074366700 @default.
- W2022227305 creator A5078103970 @default.
- W2022227305 creator A5083219290 @default.
- W2022227305 date "2007-01-01" @default.
- W2022227305 modified "2023-10-18" @default.
- W2022227305 title "Optimized amplification and fluorescent labeling of small cell samples for genomic array-CGH" @default.
- W2022227305 cites W1970980262 @default.
- W2022227305 cites W1972732916 @default.
- W2022227305 cites W1984731778 @default.
- W2022227305 cites W1986272828 @default.
- W2022227305 cites W1989215251 @default.
- W2022227305 cites W2023767070 @default.
- W2022227305 cites W2035038219 @default.
- W2022227305 cites W2037325598 @default.
- W2022227305 cites W2050289401 @default.
- W2022227305 cites W2086814185 @default.
- W2022227305 cites W2092858940 @default.
- W2022227305 cites W2097097085 @default.
- W2022227305 cites W2097348296 @default.
- W2022227305 cites W2121628365 @default.
- W2022227305 cites W2124919380 @default.
- W2022227305 cites W2145903120 @default.
- W2022227305 cites W2146531100 @default.
- W2022227305 cites W2151791969 @default.
- W2022227305 cites W2153125376 @default.
- W2022227305 cites W2154023923 @default.
- W2022227305 cites W2157450307 @default.
- W2022227305 cites W51845298 @default.
- W2022227305 doi "https://doi.org/10.1002/cyto.a.20412" @default.
- W2022227305 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17458882" @default.
- W2022227305 hasPublicationYear "2007" @default.
- W2022227305 type Work @default.
- W2022227305 sameAs 2022227305 @default.
- W2022227305 citedByCount "20" @default.
- W2022227305 countsByYear W20222273052013 @default.
- W2022227305 countsByYear W20222273052014 @default.
- W2022227305 crossrefType "journal-article" @default.
- W2022227305 hasAuthorship W2022227305A5004404120 @default.
- W2022227305 hasAuthorship W2022227305A5074366700 @default.
- W2022227305 hasAuthorship W2022227305A5078103970 @default.
- W2022227305 hasAuthorship W2022227305A5083219290 @default.
- W2022227305 hasConcept C104317684 @default.
- W2022227305 hasConcept C120821319 @default.
- W2022227305 hasConcept C124942203 @default.
- W2022227305 hasConcept C141231307 @default.
- W2022227305 hasConcept C166608930 @default.
- W2022227305 hasConcept C17757408 @default.
- W2022227305 hasConcept C54355233 @default.
- W2022227305 hasConcept C552990157 @default.
- W2022227305 hasConcept C70721500 @default.
- W2022227305 hasConcept C86803240 @default.
- W2022227305 hasConceptScore W2022227305C104317684 @default.
- W2022227305 hasConceptScore W2022227305C120821319 @default.
- W2022227305 hasConceptScore W2022227305C124942203 @default.
- W2022227305 hasConceptScore W2022227305C141231307 @default.
- W2022227305 hasConceptScore W2022227305C166608930 @default.
- W2022227305 hasConceptScore W2022227305C17757408 @default.
- W2022227305 hasConceptScore W2022227305C54355233 @default.
- W2022227305 hasConceptScore W2022227305C552990157 @default.
- W2022227305 hasConceptScore W2022227305C70721500 @default.
- W2022227305 hasConceptScore W2022227305C86803240 @default.
- W2022227305 hasIssue "8" @default.
- W2022227305 hasLocation W20222273051 @default.
- W2022227305 hasLocation W20222273052 @default.
- W2022227305 hasOpenAccess W2022227305 @default.
- W2022227305 hasPrimaryLocation W20222273051 @default.
- W2022227305 hasRelatedWork W133042624 @default.
- W2022227305 hasRelatedWork W1521296718 @default.
- W2022227305 hasRelatedWork W2038127858 @default.
- W2022227305 hasRelatedWork W2062965527 @default.
- W2022227305 hasRelatedWork W2090795400 @default.
- W2022227305 hasRelatedWork W2103025725 @default.
- W2022227305 hasRelatedWork W2113533151 @default.
- W2022227305 hasRelatedWork W2154009110 @default.
- W2022227305 hasRelatedWork W2171464739 @default.
- W2022227305 hasRelatedWork W2900674697 @default.
- W2022227305 hasVolume "71A" @default.
- W2022227305 isParatext "false" @default.
- W2022227305 isRetracted "false" @default.
- W2022227305 magId "2022227305" @default.
- W2022227305 workType "article" @default.