Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022229110> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2022229110 endingPage "609" @default.
- W2022229110 startingPage "604" @default.
- W2022229110 abstract "The action of phospholipase C (Bacillus cereus) toward mixed micelles of phosphatidylcholine and the nonionic surfactant Triton X-100 is analyzed according to the “surfaceas-cofactor” kinetic scheme recently proposed for characterizing the action of lipolytic enzymes [Deems, R. A., Eaton, B. R., and Dennis, E. A. (1975) J. Biol. Chem.250, 9013–9020]. According to this scheme, the enzyme first associates with the surface or mixed micelles, where the dissociation constant is KsA. The enzyme, now part of the mixed micelle surface, then binds the substrate phospholipid molecule in its active site and this binding is related to the Michaelis constant, KmB. The surface, or mixed micelles in this scheme, behaves kinetically as a cofactor in that, under initial rate conditions, the surface properties of the mixed micelles are virtually unchanged after catalysis. For phospholipase C with egg phosphatidylcholine as substrate, it was found that at pH 6.4 (the pH optimum for the enzyme) and 40 °C, V is about 2 × 103 μmol min−1 (mg of protein)−1. KsA is about 2 mm and KmB is 1 to 2 × 10−10 mol cm−2. The kinetic constants for phospholipase C are compared with those previously reported for phospholipase A2 and the membrane-bound enzyme phosphatidylserine decarboxylase determined under similar conditions." @default.
- W2022229110 created "2016-06-24" @default.
- W2022229110 creator A5038067245 @default.
- W2022229110 creator A5040976154 @default.
- W2022229110 date "1976-10-01" @default.
- W2022229110 modified "2023-09-27" @default.
- W2022229110 title "Analysis of phospholipase C (Bacillus cereus) action toward mixed micelles of phospholipid and surfactant" @default.
- W2022229110 cites W1522819451 @default.
- W2022229110 cites W1546753073 @default.
- W2022229110 cites W1552494303 @default.
- W2022229110 cites W1863914224 @default.
- W2022229110 cites W1966825628 @default.
- W2022229110 cites W1999163517 @default.
- W2022229110 cites W2006081418 @default.
- W2022229110 cites W2046116492 @default.
- W2022229110 cites W2048160429 @default.
- W2022229110 cites W2050906158 @default.
- W2022229110 cites W4234745691 @default.
- W2022229110 doi "https://doi.org/10.1016/0003-9861(76)90204-6" @default.
- W2022229110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10851" @default.
- W2022229110 hasPublicationYear "1976" @default.
- W2022229110 type Work @default.
- W2022229110 sameAs 2022229110 @default.
- W2022229110 citedByCount "74" @default.
- W2022229110 countsByYear W20222291102015 @default.
- W2022229110 countsByYear W20222291102016 @default.
- W2022229110 countsByYear W20222291102017 @default.
- W2022229110 countsByYear W20222291102020 @default.
- W2022229110 countsByYear W20222291102022 @default.
- W2022229110 crossrefType "journal-article" @default.
- W2022229110 hasAuthorship W2022229110A5038067245 @default.
- W2022229110 hasAuthorship W2022229110A5040976154 @default.
- W2022229110 hasConcept C11268172 @default.
- W2022229110 hasConcept C12927208 @default.
- W2022229110 hasConcept C170493617 @default.
- W2022229110 hasConcept C178790620 @default.
- W2022229110 hasConcept C181199279 @default.
- W2022229110 hasConcept C184651966 @default.
- W2022229110 hasConcept C185592680 @default.
- W2022229110 hasConcept C18903297 @default.
- W2022229110 hasConcept C2776330855 @default.
- W2022229110 hasConcept C2777118263 @default.
- W2022229110 hasConcept C2777289219 @default.
- W2022229110 hasConcept C2778918659 @default.
- W2022229110 hasConcept C41625074 @default.
- W2022229110 hasConcept C523546767 @default.
- W2022229110 hasConcept C54355233 @default.
- W2022229110 hasConcept C55493867 @default.
- W2022229110 hasConcept C58226133 @default.
- W2022229110 hasConcept C64237528 @default.
- W2022229110 hasConcept C74998103 @default.
- W2022229110 hasConcept C86803240 @default.
- W2022229110 hasConceptScore W2022229110C11268172 @default.
- W2022229110 hasConceptScore W2022229110C12927208 @default.
- W2022229110 hasConceptScore W2022229110C170493617 @default.
- W2022229110 hasConceptScore W2022229110C178790620 @default.
- W2022229110 hasConceptScore W2022229110C181199279 @default.
- W2022229110 hasConceptScore W2022229110C184651966 @default.
- W2022229110 hasConceptScore W2022229110C185592680 @default.
- W2022229110 hasConceptScore W2022229110C18903297 @default.
- W2022229110 hasConceptScore W2022229110C2776330855 @default.
- W2022229110 hasConceptScore W2022229110C2777118263 @default.
- W2022229110 hasConceptScore W2022229110C2777289219 @default.
- W2022229110 hasConceptScore W2022229110C2778918659 @default.
- W2022229110 hasConceptScore W2022229110C41625074 @default.
- W2022229110 hasConceptScore W2022229110C523546767 @default.
- W2022229110 hasConceptScore W2022229110C54355233 @default.
- W2022229110 hasConceptScore W2022229110C55493867 @default.
- W2022229110 hasConceptScore W2022229110C58226133 @default.
- W2022229110 hasConceptScore W2022229110C64237528 @default.
- W2022229110 hasConceptScore W2022229110C74998103 @default.
- W2022229110 hasConceptScore W2022229110C86803240 @default.
- W2022229110 hasIssue "2" @default.
- W2022229110 hasLocation W20222291101 @default.
- W2022229110 hasLocation W20222291102 @default.
- W2022229110 hasOpenAccess W2022229110 @default.
- W2022229110 hasPrimaryLocation W20222291101 @default.
- W2022229110 hasRelatedWork W1965121437 @default.
- W2022229110 hasRelatedWork W1981848085 @default.
- W2022229110 hasRelatedWork W2000752909 @default.
- W2022229110 hasRelatedWork W2024841440 @default.
- W2022229110 hasRelatedWork W2039850526 @default.
- W2022229110 hasRelatedWork W2054912749 @default.
- W2022229110 hasRelatedWork W2076761259 @default.
- W2022229110 hasRelatedWork W2269499905 @default.
- W2022229110 hasRelatedWork W2950315234 @default.
- W2022229110 hasRelatedWork W4253449002 @default.
- W2022229110 hasVolume "176" @default.
- W2022229110 isParatext "false" @default.
- W2022229110 isRetracted "false" @default.
- W2022229110 magId "2022229110" @default.
- W2022229110 workType "article" @default.