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• W2022229886 endingPage "74" @default.
• W2022229886 startingPage "69" @default.
• W2022229886 abstract "The present study demonstrates the presence of opioid receptors in the rat cardiac sarcolemma isolated by the hypotonic LiBr-shock procedure. Opioid binding was measured by using [3H]U69 593, [3H](2-D-penicillamine,5-D-penicillamine)enkephalin ([3H]DPDPE) or [3H][D-Ala2,MePhe4,Gly-(ol)5]enkephalin ([3H]DAGO) as selective radioligands for K, delta and mu opioid receptors, respectively. Both the K- and delta-selective ligands exhibited highly specific (75-86%) binding, saturable at a concentration of about 20 nM. No specific binding for the selective agonist DAGO was observed. A marked increase in both [3H]U69 593 and [3H]DPDPE binding was observed after incubation of the sarcolemma with the alpha-adrenoceptor agonist phenylephrine or with the beta-adrenoceptor agonist isoproterenol. These stimulatory effects were associated with an increase in the Bmax values, a decrease in the Kd values, and were completely antagonized by the respective antagonists phentolamine and propranolol." @default.
• W2022229886 created "2016-06-24" @default.
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• W2022229886 date "1989-12-01" @default.
• W2022229886 modified "2023-09-23" @default.
• W2022229886 title "Opioid receptors in rat cardiac sarcolemma: effect of phenylephrine and isoproterenol" @default.
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• W2022229886 doi "https://doi.org/10.1016/0005-2736(89)90456-2" @default.
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