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- W2022236437 abstract "This paper presents a methodology for analyzing intact Mb-size DNA which enables to obtain spatial information on the position of the specific sequence in single molecular level. We utilized the complex of Qdot-streptavidin conjugate and biotinylated PNA as a triplex forming fluorescent probe and tried to visualize a region of the origin site of chromosomal replication ( <i xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>ori</i> C) on an intact genomic DNA of a hyperthermophilic archaeon, <i xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>Thermococcus</i> <i xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>kodakaraensis</i> KOD1. To improve the spatial resolution along the DNA, the DNA was dragged and elongated in the solution by the optical tweezers. PNA binding to homopurine sequence in <i xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>ori</i> C region of KOD1 was confirmed by the gel electrophoresis mobility shift assay and fluorescence optical mapping in single molecular level was demonstrated. However, we have not yet detected the <i xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>ori</i> C region specifically due to the difficulty of the complete elimination of the non-specific binding between Qdot-streptavidin conjugate and DNA." @default.
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- W2022236437 date "2007-11-01" @default.
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- W2022236437 title "Visualization of an oriC region on an isolated single whole-genome DNA with triplex forming PNA probe using fluorescence microscopy" @default.
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- W2022236437 doi "https://doi.org/10.1109/mhs.2007.4420830" @default.
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