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• W2022238272 abstract "Two types of phospholipid vesicles capable of mutual recognition have been tailor-made to serve as a model system for the study of carbohydrate-mediated cellular adhesion. One of the vesicles contained a fatty acid conjugate of a galactose specific lectin (lectin vesicle_ and the other an asialoganglioside with a reactive terminal galactose residue (galactose vesicle). The kinetics of aggregation of these two types of vesicles was followed by monitoring time-dependent change in turbidity. A 10–100-fold enhancement in the forward rate constant (kf ranging from 7.1 · 105 to 4.5 · 107 M−1 · s−1 at 27° C) was observed when compared with that for the lectin-galactose system in solution (kt) being 4.5 · 105 M−1 · s−1), reported in the literature. A study of the influence of vesicle size on the rate of aggregation showed that enhancement depended on the curvature of the galactose vesicle rather than the density of asialoganglioside suggesting a possible diffusion in the plane of the membrane. The ratio, kf/kd is found to be approx. 1010 M−1 indicating that the formation of multiple bonds plays a role for stable adhesion." @default.
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• W2022238272 date "1990-05-01" @default.
• W2022238272 modified "2023-09-29" @default.
• W2022238272 title "Complex carbohydrate-lectin interaction at the interface: a model for cellular adhesion. I. Effect of vesicle size on the kinetics of aggregation between a fatty acid conjugate of lectin and a liposomal asialoganglioside" @default.
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• W2022238272 doi "https://doi.org/10.1016/0005-2736(90)90213-8" @default.
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