Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022240674> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2022240674 endingPage "S116" @default.
- W2022240674 startingPage "S110" @default.
- W2022240674 abstract "Toxic shock syndrome toxin 1 (TSST-1) was partially purified from culture supernatants by SP-Sephadex C-25 ion-exchange chromatography and subsequent Sephadex G-75 gel filtration. This protein had an apparent molecular weight of 24,000 and an isoelectric point of 7.0. The NH2-amino acid sequence for the first 40 residues agreed completely with that predicted from the known TSST-1 genome. Ouchterlony immunodiffusion with monospecific rabbit antisera demonstrated a single line of identity with reference TSST-1 as well as with three preparations obtained from other investigators. When the purity of the different TSST-1 preparations was examined by Coomassie blue or silver staining after SDS-PAGE, only the major band at molecular weight 24,000 was apparent. However, multiple additional bands were seen in all preparations when visualized either by double staining with Coomassie blue stain followed by silver stain or by immunoblot using pooled human serum. Further purification of our preparation by reverse-phase high-performance liquid chromatography eliminated some, but not all, extraneous antigens. A final purification step by preparative SDS-PAGE resulted in an eluted protein that yielded only the 24-kDa TSST-1 band and a 48-kDa dimer by immunoblot. This material was endotoxin free (sensitivity limit, 10 pg/mL) and retained biologic activity for induction of cachectin and production of interleukin 1 by human monocytes. These data emphasize the need for stringent methods of assessment of purity in TSST-1 preparations." @default.
- W2022240674 created "2016-06-24" @default.
- W2022240674 creator A5024039073 @default.
- W2022240674 creator A5049205325 @default.
- W2022240674 creator A5061912019 @default.
- W2022240674 date "1989-01-01" @default.
- W2022240674 modified "2023-10-18" @default.
- W2022240674 title "Purification and Purity Assessment of Toxic Shock Syndrome Toxin 1" @default.
- W2022240674 cites W1508459991 @default.
- W2022240674 cites W167158724 @default.
- W2022240674 cites W1775749144 @default.
- W2022240674 cites W1870087846 @default.
- W2022240674 cites W1873126998 @default.
- W2022240674 cites W1898745538 @default.
- W2022240674 cites W1915062162 @default.
- W2022240674 cites W1980106007 @default.
- W2022240674 cites W2011250547 @default.
- W2022240674 cites W2011333426 @default.
- W2022240674 cites W2023129826 @default.
- W2022240674 cites W2059059013 @default.
- W2022240674 cites W2100837269 @default.
- W2022240674 cites W2122035318 @default.
- W2022240674 cites W2172140803 @default.
- W2022240674 cites W2209326117 @default.
- W2022240674 cites W2416056314 @default.
- W2022240674 doi "https://doi.org/10.1093/clinids/11.supplement_1.s110" @default.
- W2022240674 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2494689" @default.
- W2022240674 hasPublicationYear "1989" @default.
- W2022240674 type Work @default.
- W2022240674 sameAs 2022240674 @default.
- W2022240674 citedByCount "10" @default.
- W2022240674 countsByYear W20222406742013 @default.
- W2022240674 crossrefType "journal-article" @default.
- W2022240674 hasAuthorship W2022240674A5024039073 @default.
- W2022240674 hasAuthorship W2022240674A5049205325 @default.
- W2022240674 hasAuthorship W2022240674A5061912019 @default.
- W2022240674 hasConcept C147483822 @default.
- W2022240674 hasConcept C153911025 @default.
- W2022240674 hasConcept C163994747 @default.
- W2022240674 hasConcept C181199279 @default.
- W2022240674 hasConcept C185592680 @default.
- W2022240674 hasConcept C193363816 @default.
- W2022240674 hasConcept C196955773 @default.
- W2022240674 hasConcept C203014093 @default.
- W2022240674 hasConcept C25330733 @default.
- W2022240674 hasConcept C2777367657 @default.
- W2022240674 hasConcept C2780761556 @default.
- W2022240674 hasConcept C43617362 @default.
- W2022240674 hasConcept C50476208 @default.
- W2022240674 hasConcept C54355233 @default.
- W2022240674 hasConcept C55493867 @default.
- W2022240674 hasConcept C74864618 @default.
- W2022240674 hasConcept C80020023 @default.
- W2022240674 hasConcept C86803240 @default.
- W2022240674 hasConcept C91523082 @default.
- W2022240674 hasConcept C96294017 @default.
- W2022240674 hasConceptScore W2022240674C147483822 @default.
- W2022240674 hasConceptScore W2022240674C153911025 @default.
- W2022240674 hasConceptScore W2022240674C163994747 @default.
- W2022240674 hasConceptScore W2022240674C181199279 @default.
- W2022240674 hasConceptScore W2022240674C185592680 @default.
- W2022240674 hasConceptScore W2022240674C193363816 @default.
- W2022240674 hasConceptScore W2022240674C196955773 @default.
- W2022240674 hasConceptScore W2022240674C203014093 @default.
- W2022240674 hasConceptScore W2022240674C25330733 @default.
- W2022240674 hasConceptScore W2022240674C2777367657 @default.
- W2022240674 hasConceptScore W2022240674C2780761556 @default.
- W2022240674 hasConceptScore W2022240674C43617362 @default.
- W2022240674 hasConceptScore W2022240674C50476208 @default.
- W2022240674 hasConceptScore W2022240674C54355233 @default.
- W2022240674 hasConceptScore W2022240674C55493867 @default.
- W2022240674 hasConceptScore W2022240674C74864618 @default.
- W2022240674 hasConceptScore W2022240674C80020023 @default.
- W2022240674 hasConceptScore W2022240674C86803240 @default.
- W2022240674 hasConceptScore W2022240674C91523082 @default.
- W2022240674 hasConceptScore W2022240674C96294017 @default.
- W2022240674 hasIssue "Supplement_1" @default.
- W2022240674 hasLocation W20222406741 @default.
- W2022240674 hasLocation W20222406742 @default.
- W2022240674 hasOpenAccess W2022240674 @default.
- W2022240674 hasPrimaryLocation W20222406741 @default.
- W2022240674 hasRelatedWork W1804075811 @default.
- W2022240674 hasRelatedWork W1980106007 @default.
- W2022240674 hasRelatedWork W2022240674 @default.
- W2022240674 hasRelatedWork W2047585996 @default.
- W2022240674 hasRelatedWork W2064680021 @default.
- W2022240674 hasRelatedWork W2070282372 @default.
- W2022240674 hasRelatedWork W2090162049 @default.
- W2022240674 hasRelatedWork W2170656861 @default.
- W2022240674 hasRelatedWork W2332711745 @default.
- W2022240674 hasRelatedWork W2413941662 @default.
- W2022240674 hasVolume "11" @default.
- W2022240674 isParatext "false" @default.
- W2022240674 isRetracted "false" @default.
- W2022240674 magId "2022240674" @default.
- W2022240674 workType "article" @default.