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- W2022245087 abstract "Abstract The chemical synthesis of [ 3 H]-7-dehydrocholesterol from [ 3 H]-cholesterol is described and the incorporation of [ 14 C]-cholesterol, [ 3 H]-7-dehydrocholesterol and [ 3 H]-5α-cholest-7-en-3β-ol into β-ecdysone in the locust, Schistocerca gregaria , have been compared. The ratio of incorporation into β-ecdysone of [ 3 H]-7-dehydrocholesterol to that of [ 14 C]-cholesterol administered simultaneously was 0.97:1. When these substrates were administered in the presence of non-radioactive 7-dehydrocholesterol this ratio was increased to 1.80:1. The incorporation of [ 3 H]-5α-cholest-7-en-3β-ol into β-ecdysone was low (0.01%) compared with that of cholesterol (0.12%), suggesting that the former sterol is not a normal intermediate in ecdysone biosynthesis. The results are discussed in relation to the role of 7-dehydrocholesterol in ecdysone biosynthesis. Analysis of the radioactive sterols indicated that cholesterol, 7-dehydrocholesterol, 5α-cholest-7-en-3β-ol and 5α-cholestan-3β-ol are interconvertible, and that there is a large conversion of 7-dehydrocholesterol into cholesterol." @default.
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- W2022245087 title "Biosynthesis of ecdysones: Metabolism of 7-dehydrocholesterol in Schistocerca gregaria" @default.
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