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- W2022251251 abstract "Optical tweezers are well-known tools for the mechanical manipulation of single molecules in aqueous solutions. Here Iwill discuss a new development - the combination of optical tweezers with solid-state nanopores. Nanopores are holes inthin membranes usually a few 10s of nm in diameter or even with single nm diameters. In aqueous solutions an ioniccurrent can be driven through a nanopore and thus the translocation of a single molecule detected. Although thisinformation can be used to characterize the length and charge of the molecules, there is no information about the force orposition during this process. I will discuss how optical tweezers can be used to mechanically control the translocationprocess, what we learned so far and where we are going with the technology. In particular, I will show that the opticaltweezers/nanopore combination proved to be of exceptional value in unraveling the coupling between electrokinetic andhydrodynamic effects during voltage-driven translocation. This has implication for a wide range of applications rangingfrom gel electrophoresis to DNA manipulation for lab-on-the chip technology." @default.
- W2022251251 created "2016-06-24" @default.
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- W2022251251 date "2010-08-19" @default.
- W2022251251 modified "2023-09-23" @default.
- W2022251251 title "DNA tug of war: tether forces on DNA in a nanopore" @default.
- W2022251251 doi "https://doi.org/10.1117/12.862650" @default.
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