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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022254501> ?p ?o ?g. }

• W2022254501 abstract "An IgM antibody was purified from hybridoma supernatant containing serum using a three-step purification process comprising of tangential flow filtration, anion-exchange chromatography and size-exclusion chromatography. Recovery and purity were significantly improved upon adaptation of the hybridoma to serum-free media. The process could even be simplified by omitting the initial tangential flow filtration step. Even with a two-step purification process a purity of >98% and a recovery of >60% was obtained." @default.
• W2022254501 created "2016-06-24" @default.
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• W2022254501 date "2009-03-01" @default.
• W2022254501 modified "2023-09-25" @default.
• W2022254501 title "Adaptation of hybridomas to protein-free media results in a simplified two-step immunoglobulin M purification process" @default.
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• W2022254501 doi "https://doi.org/10.1016/j.chroma.2008.10.067" @default.
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