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• W2022265488 abstract "Using a fixed cell ELISA system E-selectin expression and adhesion of U937 cells were measured in both endotoxin (LPS: serotype 0111:B4) tregted and untreated cultures of human umbilical vein endothelial cells (HUVEC). Basal levels of E-selectin were unde-tectable whereas significant induction of both E-selectin and U937 cell adhesion were observed after exposure of HUVEC to LPS for 6h. The effect of LPS was serum dependent and unaffected by coincubation with neutralising antibodies to either IL-1α, IL-1β or TNFα either alone or in combination, even though each antibody was capable of neutralising the effect of exogenously added cytokine. In addition IgG fractions of neutralising anti-cytokine antibodies were purified, concentrated and microinjected into the cytosol of adherent HUVEC prior to treatment with 1 μg/ml LPS for 6 hrs. Immunofluorescence staining showed that cells microinjected with antibodies to IL-1α, IL-1β and TNFα either alone or in combination were positive for LPS-stimulated E-selectin, demonstrating the action of LPS is not mediated by intracellular action of cytokines. The action of LPS on endothelial E-selectin induction or adhesion of U937 cells was not increased either by coincubation with IFN-y or by pretreatment with IFN-γ 96 hrs prior to stimulation with LPS. LPS activity was not mediated by bradykinin, platelet activating factor, IL-6 or IL-8 since these molecules did not induce endothelial cell E-selectin expression. The action of LPS on HUVEC is attributed to a direct effect since treatment of HUVEC with conditioned medium from LPS-treated HUVEC in which polymyxin B was added to neutralise the LPS, was ineffective in inducing E-selectin expression. However conditioned medium from LPS treated U937 cells was able to induce E-selectin expression on previously unstimulated HUVEC, an effect which could be blocked by coincubation of conditioned medium with neutralising anti-TNFα antibody." @default.
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• W2022265488 date "1995-01-01" @default.
• W2022265488 modified "2023-09-28" @default.
• W2022265488 title "Induction of E-Selectin by Endotoxin is Direct and not Mediated Through Intracellular or Extracellular Release of Secondary Cytokines" @default.
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• W2022265488 doi "https://doi.org/10.3109/10623329509024665" @default.
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