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• W2022271802 abstract "Transforming growth factor-β1 increases albumin permeability of isolated rat glomeruli via hydroxyl radicals.BackgroundTransforming growth factor-β1 (TGF-β1) is a multifunctional cytokine. Glomerular cells and tubular epithelial cells secrete and respond to TGF-β1. A close association between elevated levels of TGF-β1 and the development of glomerulonephritis, glomerulosclerosis, and tubular hypertrophy has been documented. The role of TGF-β1 in proteinuria is not well understood.MethodsIsolated rat glomeruli were incubated in medium alone or with TGF-β1 (1 to 10 ng/mL) and TGF-β1 + 200 U/mL of superoxide dismutase (SOD) or 1 mmol/L of dimethylthiourea (DMTU) scavengers of superoxide and hydroxyl radicals, respectively, for up to 60 minutes at 37°C. Glomerular albumin permeability (Palb) was calculated from the volumetric response of glomeruli to an oncotic gradient using videomicroscopy.ResultsOne or 2.5 ng/mL of TGF-β1 had no effect on Palb (0.18 ± 0.08, N = 17; 0.18 ± 0.079, N = 20 vs. control 0.00 ± 0.06, N = 25), whereas 5 or 10 ng/mL of TGF-β1 caused a significant increase in Palb (0.31 ± 0.09, N = 20; 0.33 ± 0.06, N = 23) within 15 minutes. The effect of 10 ng/mL of TGF-β1 on Palb increased further after 30, 45, or 60 minutes of incubation (0.43 ± 0.06, N = 24; 0.53 ± 0.06, N = 25; 0.74 ± 0.075, N = 21). The TGF-β1–induced increase in Palb (0.75 ± 0.065, N = 15) was blocked by SOD (0.07 ± 0.14 N = 15) or by DMTU (0.04 ± 0.13, N = 15). Incubation of glomeruli with the carrier medium (4N HCl) in which TGF-β1 is dissolved and SOD or DMTU alone did not affect Palb.ConclusionElevated levels of TGF-β1 derived from glomerular or extraglomerular sources are capable of increasing glomerular Palb via superoxide and hydroxyl radicals and may lead to proteinuria in vivo. Transforming growth factor-β1 increases albumin permeability of isolated rat glomeruli via hydroxyl radicals. Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine. Glomerular cells and tubular epithelial cells secrete and respond to TGF-β1. A close association between elevated levels of TGF-β1 and the development of glomerulonephritis, glomerulosclerosis, and tubular hypertrophy has been documented. The role of TGF-β1 in proteinuria is not well understood. Isolated rat glomeruli were incubated in medium alone or with TGF-β1 (1 to 10 ng/mL) and TGF-β1 + 200 U/mL of superoxide dismutase (SOD) or 1 mmol/L of dimethylthiourea (DMTU) scavengers of superoxide and hydroxyl radicals, respectively, for up to 60 minutes at 37°C. Glomerular albumin permeability (Palb) was calculated from the volumetric response of glomeruli to an oncotic gradient using videomicroscopy. One or 2.5 ng/mL of TGF-β1 had no effect on Palb (0.18 ± 0.08, N = 17; 0.18 ± 0.079, N = 20 vs. control 0.00 ± 0.06, N = 25), whereas 5 or 10 ng/mL of TGF-β1 caused a significant increase in Palb (0.31 ± 0.09, N = 20; 0.33 ± 0.06, N = 23) within 15 minutes. The effect of 10 ng/mL of TGF-β1 on Palb increased further after 30, 45, or 60 minutes of incubation (0.43 ± 0.06, N = 24; 0.53 ± 0.06, N = 25; 0.74 ± 0.075, N = 21). The TGF-β1–induced increase in Palb (0.75 ± 0.065, N = 15) was blocked by SOD (0.07 ± 0.14 N = 15) or by DMTU (0.04 ± 0.13, N = 15). Incubation of glomeruli with the carrier medium (4N HCl) in which TGF-β1 is dissolved and SOD or DMTU alone did not affect Palb. Elevated levels of TGF-β1 derived from glomerular or extraglomerular sources are capable of increasing glomerular Palb via superoxide and hydroxyl radicals and may lead to proteinuria in vivo." @default.
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• W2022271802 date "2000-07-01" @default.
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• W2022271802 title "Transforming growth factor-β1 increases albumin permeability of isolated rat glomeruli via hydroxyl radicals" @default.
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• W2022271802 doi "https://doi.org/10.1046/j.1523-1755.2000.00148.x" @default.
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