FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022281569> ?p ?o ?g. }

• W2022281569 endingPage "246" @default.
• W2022281569 startingPage "239" @default.
• W2022281569 abstract "Zebrafish have recently become a model of choice among developmental biologists. This unique model enables both modern molecular and genetic studies to be carried out to identify genes involved in a wide variety of developmental processes. The success of the genetic approach depends largely on the application of an easy and effective screening method to identify interesting mutants. In order to develop a method for visualizing skeletal structures in zebrafish embryos that would be suitable for screening skeletal mutants, we investigated the use of the fluorescent chromophore calcein, which binds specifically to calcified skeletal structures. By using this method, we followed the development of the skeletal structures in zebrafish embryos from day 1 to day 21 postfertilization, and analyzed the effect of bone morphogenetic protein-2 (BMP2) on axial skeleton development. We found the development of the calcified skeletal structure to appear in a progressive fashion from head to tail. Calcified structures in the head (i.e., the jaw) developed first, which were then followed by the axial skeleton in the trunk. Interesting to note was that there appeared to be two domains in the calcification of vertebrae within the axial skeleton. The first three vertebrae were in the first domain; the rest being in the second domain. Compared with Alcian blue staining, we found that calcein staining indeed labels calcified skeletal structures, and, moreover, it is a more sensitive and inclusive method for visualizing skeletal structures. To determine whether calcein staining could also be used to detect abnormal bone development, we ectopically expressed BMP2 in zebrafish notochord cells. We demonstrated that ectopic expression of BMP2 in notochord cells inhibited the development of the axial skeleton. Together, these results clearly demonstrated the sensitivity of calcein staining for visualizing bone structures in developing zebrafish embryos and its effectiveness for screening for mutants that have bone structure defects." @default.
• W2022281569 created "2016-06-24" @default.
• W2022281569 creator A5013773800 @default.
• W2022281569 creator A5033770270 @default.
• W2022281569 creator A5063849560 @default.
• W2022281569 creator A5070288672 @default.
• W2022281569 date "2001-10-01" @default.
• W2022281569 modified "2023-10-10" @default.
• W2022281569 title "Visualizing Normal and Defective Bone Development in Zebrafish Embryos Using the Fluorescent Chromophore Calcein" @default.
• W2022281569 cites W1509868604 @default.
• W2022281569 cites W1539975895 @default.
• W2022281569 cites W1834486239 @default.
• W2022281569 cites W1932232911 @default.
• W2022281569 cites W1958621441 @default.
• W2022281569 cites W1980276935 @default.
• W2022281569 cites W1986469118 @default.
• W2022281569 cites W1993950414 @default.
• W2022281569 cites W1994557963 @default.
• W2022281569 cites W2006950202 @default.
• W2022281569 cites W2007812207 @default.
• W2022281569 cites W2011180667 @default.
• W2022281569 cites W2013734851 @default.
• W2022281569 cites W2022906661 @default.
• W2022281569 cites W2025019677 @default.
• W2022281569 cites W2028260634 @default.
• W2022281569 cites W2034715594 @default.
• W2022281569 cites W2055110202 @default.
• W2022281569 cites W2065200502 @default.
• W2022281569 cites W2077559627 @default.
• W2022281569 cites W2093925575 @default.
• W2022281569 cites W2101821342 @default.
• W2022281569 cites W2123938765 @default.
• W2022281569 cites W2125349610 @default.
• W2022281569 cites W2125551434 @default.
• W2022281569 cites W2142524309 @default.
• W2022281569 cites W4240053492 @default.
• W2022281569 doi "https://doi.org/10.1006/dbio.2001.0390" @default.
• W2022281569 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11784007" @default.
• W2022281569 hasPublicationYear "2001" @default.
• W2022281569 type Work @default.
• W2022281569 sameAs 2022281569 @default.
• W2022281569 citedByCount "228" @default.
• W2022281569 countsByYear W20222815692012 @default.
• W2022281569 countsByYear W20222815692013 @default.
• W2022281569 countsByYear W20222815692014 @default.
• W2022281569 countsByYear W20222815692015 @default.
• W2022281569 countsByYear W20222815692016 @default.
• W2022281569 countsByYear W20222815692017 @default.
• W2022281569 countsByYear W20222815692018 @default.
• W2022281569 countsByYear W20222815692019 @default.
• W2022281569 countsByYear W20222815692020 @default.
• W2022281569 countsByYear W20222815692021 @default.
• W2022281569 countsByYear W20222815692022 @default.
• W2022281569 countsByYear W20222815692023 @default.
• W2022281569 crossrefType "journal-article" @default.
• W2022281569 hasAuthorship W2022281569A5013773800 @default.
• W2022281569 hasAuthorship W2022281569A5033770270 @default.
• W2022281569 hasAuthorship W2022281569A5063849560 @default.
• W2022281569 hasAuthorship W2022281569A5070288672 @default.
• W2022281569 hasBestOaLocation W20222815691 @default.
• W2022281569 hasConcept C104317684 @default.
• W2022281569 hasConcept C105702510 @default.
• W2022281569 hasConcept C142724271 @default.
• W2022281569 hasConcept C18969341 @default.
• W2022281569 hasConcept C2776872141 @default.
• W2022281569 hasConcept C2776878037 @default.
• W2022281569 hasConcept C2777971045 @default.
• W2022281569 hasConcept C2780309369 @default.
• W2022281569 hasConcept C2910894859 @default.
• W2022281569 hasConcept C31507581 @default.
• W2022281569 hasConcept C41625074 @default.
• W2022281569 hasConcept C54355233 @default.
• W2022281569 hasConcept C71924100 @default.
• W2022281569 hasConcept C74864618 @default.
• W2022281569 hasConcept C86803240 @default.
• W2022281569 hasConcept C95444343 @default.
• W2022281569 hasConceptScore W2022281569C104317684 @default.
• W2022281569 hasConceptScore W2022281569C105702510 @default.
• W2022281569 hasConceptScore W2022281569C142724271 @default.
• W2022281569 hasConceptScore W2022281569C18969341 @default.
• W2022281569 hasConceptScore W2022281569C2776872141 @default.
• W2022281569 hasConceptScore W2022281569C2776878037 @default.
• W2022281569 hasConceptScore W2022281569C2777971045 @default.
• W2022281569 hasConceptScore W2022281569C2780309369 @default.
• W2022281569 hasConceptScore W2022281569C2910894859 @default.
• W2022281569 hasConceptScore W2022281569C31507581 @default.
• W2022281569 hasConceptScore W2022281569C41625074 @default.
• W2022281569 hasConceptScore W2022281569C54355233 @default.
• W2022281569 hasConceptScore W2022281569C71924100 @default.
• W2022281569 hasConceptScore W2022281569C74864618 @default.
• W2022281569 hasConceptScore W2022281569C86803240 @default.
• W2022281569 hasConceptScore W2022281569C95444343 @default.
• W2022281569 hasIssue "2" @default.
• W2022281569 hasLocation W20222815691 @default.
• W2022281569 hasLocation W20222815692 @default.
• W2022281569 hasOpenAccess W2022281569 @default.
• W2022281569 hasPrimaryLocation W20222815691 @default.
• W2022281569 hasRelatedWork W1964736352 @default.

• next