FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022287411> ?p ?o ?g. }

• W2022287411 endingPage "1179" @default.
• W2022287411 startingPage "1173" @default.
• W2022287411 abstract "This protocol details methods for the generation of cell-free extracts and DNA templates from the eggs and sperm chromatin, respectively, of the clawed toad Xenopus laevis. We have used this system with scanning electron microscopy (SEM), as detailed herein, to analyze the biochemical requirements and structural pathways for the biogenesis of eukaryotic nuclear envelopes (NEs) and nuclear pore complexes (NPCs). This protocol requires access to female frogs, which are induced to lay eggs, and a male frog, which is killed for preparation of the sperm chromatin. Egg extracts should be prepared in 1 d and can be stored for many months at -80 degrees C. Demembranated sperm chromatin should take only approximately 2-3 h to prepare and can be stored at -80 degrees C almost indefinitely. The time required for assembly of structurally and functionally competent nuclei in vitro depends largely on the quality of the cell-free extracts and, therefore, must be determined for each extract preparation." @default.
• W2022287411 created "2016-06-24" @default.
• W2022287411 creator A5002019350 @default.
• W2022287411 creator A5008346318 @default.
• W2022287411 creator A5015008499 @default.
• W2022287411 creator A5027975124 @default.
• W2022287411 creator A5037898159 @default.
• W2022287411 creator A5042629715 @default.
• W2022287411 creator A5058278127 @default.
• W2022287411 creator A5086222423 @default.
• W2022287411 date "2007-05-01" @default.
• W2022287411 modified "2023-10-02" @default.
• W2022287411 title "Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro–formed nuclei for Western blotting and scanning electron microscopy (SEM)" @default.
• W2022287411 cites W1420696562 @default.
• W2022287411 cites W1489293129 @default.
• W2022287411 cites W1927520851 @default.
• W2022287411 cites W1978450158 @default.
• W2022287411 cites W1978583145 @default.
• W2022287411 cites W2011238489 @default.
• W2022287411 cites W2013801092 @default.
• W2022287411 cites W2020212714 @default.
• W2022287411 cites W2045082605 @default.
• W2022287411 cites W2045564536 @default.
• W2022287411 cites W2122028420 @default.
• W2022287411 cites W2409305791 @default.
• W2022287411 cites W2413649936 @default.
• W2022287411 cites W73930124 @default.
• W2022287411 doi "https://doi.org/10.1038/nprot.2007.138" @default.
• W2022287411 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17546012" @default.
• W2022287411 hasPublicationYear "2007" @default.
• W2022287411 type Work @default.
• W2022287411 sameAs 2022287411 @default.
• W2022287411 citedByCount "13" @default.
• W2022287411 countsByYear W20222874112012 @default.
• W2022287411 countsByYear W20222874112013 @default.
• W2022287411 countsByYear W20222874112014 @default.
• W2022287411 countsByYear W20222874112015 @default.
• W2022287411 countsByYear W20222874112016 @default.
• W2022287411 countsByYear W20222874112023 @default.
• W2022287411 crossrefType "journal-article" @default.
• W2022287411 hasAuthorship W2022287411A5002019350 @default.
• W2022287411 hasAuthorship W2022287411A5008346318 @default.
• W2022287411 hasAuthorship W2022287411A5015008499 @default.
• W2022287411 hasAuthorship W2022287411A5027975124 @default.
• W2022287411 hasAuthorship W2022287411A5037898159 @default.
• W2022287411 hasAuthorship W2022287411A5042629715 @default.
• W2022287411 hasAuthorship W2022287411A5058278127 @default.
• W2022287411 hasAuthorship W2022287411A5086222423 @default.
• W2022287411 hasBestOaLocation W20222874111 @default.
• W2022287411 hasConcept C104317684 @default.
• W2022287411 hasConcept C12554922 @default.
• W2022287411 hasConcept C126725582 @default.
• W2022287411 hasConcept C153911025 @default.
• W2022287411 hasConcept C18903297 @default.
• W2022287411 hasConcept C190062978 @default.
• W2022287411 hasConcept C202751555 @default.
• W2022287411 hasConcept C2778166480 @default.
• W2022287411 hasConcept C2779535977 @default.
• W2022287411 hasConcept C2781087480 @default.
• W2022287411 hasConcept C54355233 @default.
• W2022287411 hasConcept C552990157 @default.
• W2022287411 hasConcept C83640560 @default.
• W2022287411 hasConcept C86803240 @default.
• W2022287411 hasConcept C95444343 @default.
• W2022287411 hasConceptScore W2022287411C104317684 @default.
• W2022287411 hasConceptScore W2022287411C12554922 @default.
• W2022287411 hasConceptScore W2022287411C126725582 @default.
• W2022287411 hasConceptScore W2022287411C153911025 @default.
• W2022287411 hasConceptScore W2022287411C18903297 @default.
• W2022287411 hasConceptScore W2022287411C190062978 @default.
• W2022287411 hasConceptScore W2022287411C202751555 @default.
• W2022287411 hasConceptScore W2022287411C2778166480 @default.
• W2022287411 hasConceptScore W2022287411C2779535977 @default.
• W2022287411 hasConceptScore W2022287411C2781087480 @default.
• W2022287411 hasConceptScore W2022287411C54355233 @default.
• W2022287411 hasConceptScore W2022287411C552990157 @default.
• W2022287411 hasConceptScore W2022287411C83640560 @default.
• W2022287411 hasConceptScore W2022287411C86803240 @default.
• W2022287411 hasConceptScore W2022287411C95444343 @default.
• W2022287411 hasIssue "5" @default.
• W2022287411 hasLocation W20222874111 @default.
• W2022287411 hasLocation W20222874112 @default.
• W2022287411 hasOpenAccess W2022287411 @default.
• W2022287411 hasPrimaryLocation W20222874111 @default.
• W2022287411 hasRelatedWork W1976957428 @default.
• W2022287411 hasRelatedWork W2001442551 @default.
• W2022287411 hasRelatedWork W2015146829 @default.
• W2022287411 hasRelatedWork W2022287411 @default.
• W2022287411 hasRelatedWork W2042193656 @default.
• W2022287411 hasRelatedWork W2053302830 @default.
• W2022287411 hasRelatedWork W2169136541 @default.
• W2022287411 hasRelatedWork W2183681209 @default.
• W2022287411 hasRelatedWork W2363818879 @default.
• W2022287411 hasRelatedWork W4251371295 @default.
• W2022287411 hasVolume "2" @default.
• W2022287411 isParatext "false" @default.
• W2022287411 isRetracted "false" @default.
• W2022287411 magId "2022287411" @default.

• next