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- W2022293675 abstract "The morphoregulatory effect of FeEDTA chelating complex was verified in an androgenically more productive cultivar of tobacco White Burley and inDatura innoxia Mill. The presence of EDTA in Nitsch (1969) medium (N medium) was sufficient for the conversion of isolated embryoids and embryoids in anthers into complete plants. The androgenic development was most rapid in N medium with FeEDTA. In N medium with EDTA, the cultures developed more slowly and remained vital for a long time. In the medium without EDTA, new embryoids developed continuously. The embryoids arose and developed also in closed anthers. All embryoids which arose in anthers cultured on N medium were capable of forming complete plants. The threshold morphoregulatory concentration of FeEDTA was 40 μM FeEDTA per 1 litre of medium for embryoids in anthers and 30 μM FeEDTA per 1 litre for isolated embryoids. The dark brown colour of the anthers of tobacco andD. innoxia was due to the presence of iron (FeEDTA) in the culture medium. The morphoregulatory effect of EDTA in N medium is explained by the formation of a complex with iron present in the form of pollution of chemicals and agar. This amount of iron in complex with EDTA is sufficient for the conversion of embryoids into plants, but it is insufficient for the browning of anthers." @default.
- W2022293675 created "2016-06-24" @default.
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- W2022293675 date "1982-07-01" @default.
- W2022293675 modified "2023-09-23" @default.
- W2022293675 title "In vitro regulation of androgenesis by iron ions and chelate: A common property of two androgenic species (Nicotiana tabacum L. andDatura innoxia Mill.)" @default.
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- W2022293675 doi "https://doi.org/10.1007/bf02879462" @default.
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