FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022295532> ?p ?o ?g. }

• W2022295532 endingPage "25590" @default.
• W2022295532 startingPage "25584" @default.
• W2022295532 abstract "Human TNF-stimulated gene 14 (TSG-14) encodes a secreted 42-kDa glycoprotein that shows significant homology to proteins of the pentraxin family, which includes the acute phase reactants C-reactive protein and serum amyloid P component. Levels of TSG-14 protein (also termed PTX-3) become elevated in the serum of mice and humans after injection with bacterial lipopolysaccharide, but in contrast to conventional acute phase proteins, the bulk of TSG-14 synthesis in the intact organism occurs outside the liver. In the present study we cloned and partially sequenced murine genomic TSG-14 DNA. Analysis of the coding region predicts a high degree of amino acid sequence homology between murine and human TSG-14 (88 and 75% identity in the first and second exons, respectively). The promoter of the TSG-14 gene lacks consensus sequences for either a TATA box or CCAAT box. Primer extension analysis and S1 nuclease protection assay revealed one major transcription start site, situated within a consensus sequence for an initiator element. Sequence analysis of a ˜1.4-kilobase pair fragment of the 5′-flanking region of the TSG-14 gene revealed the presence of numerous potential enhancer binding elements, including six NF-IL6-like sites, four AP-1, one AP-2, one NF-κB, two Sp1, two interferon-γ-activated sites (GAS), one Hox-1.3, and five binding sites for Ets family members. Transfection of BALB/c 3T3 cells with promoter DNA fragments linked to the luciferase reporter gene revealed that the 5′-flanking region of the TSG-14 gene comprises elements that can mediate a basal level of transcription and inducibility by TNF. Human TNF-stimulated gene 14 (TSG-14) encodes a secreted 42-kDa glycoprotein that shows significant homology to proteins of the pentraxin family, which includes the acute phase reactants C-reactive protein and serum amyloid P component. Levels of TSG-14 protein (also termed PTX-3) become elevated in the serum of mice and humans after injection with bacterial lipopolysaccharide, but in contrast to conventional acute phase proteins, the bulk of TSG-14 synthesis in the intact organism occurs outside the liver. In the present study we cloned and partially sequenced murine genomic TSG-14 DNA. Analysis of the coding region predicts a high degree of amino acid sequence homology between murine and human TSG-14 (88 and 75% identity in the first and second exons, respectively). The promoter of the TSG-14 gene lacks consensus sequences for either a TATA box or CCAAT box. Primer extension analysis and S1 nuclease protection assay revealed one major transcription start site, situated within a consensus sequence for an initiator element. Sequence analysis of a ˜1.4-kilobase pair fragment of the 5′-flanking region of the TSG-14 gene revealed the presence of numerous potential enhancer binding elements, including six NF-IL6-like sites, four AP-1, one AP-2, one NF-κB, two Sp1, two interferon-γ-activated sites (GAS), one Hox-1.3, and five binding sites for Ets family members. Transfection of BALB/c 3T3 cells with promoter DNA fragments linked to the luciferase reporter gene revealed that the 5′-flanking region of the TSG-14 gene comprises elements that can mediate a basal level of transcription and inducibility by TNF." @default.
• W2022295532 created "2016-06-24" @default.
• W2022295532 creator A5004616732 @default.
• W2022295532 creator A5031770387 @default.
• W2022295532 creator A5046740981 @default.
• W2022295532 creator A5070227595 @default.
• W2022295532 date "1995-10-01" @default.
• W2022295532 modified "2023-09-29" @default.
• W2022295532 title "Promoter Structure and Transcriptional Activation of the Murine TSG-14 Gene Encoding a Tumor Necrosis Factor/Interleukin-1-inducible Pentraxin Protein" @default.
• W2022295532 cites W1499477548 @default.
• W2022295532 cites W1514079210 @default.
• W2022295532 cites W1514303750 @default.
• W2022295532 cites W1537516218 @default.
• W2022295532 cites W1539151577 @default.
• W2022295532 cites W1540456652 @default.
• W2022295532 cites W1563249633 @default.
• W2022295532 cites W1679413993 @default.
• W2022295532 cites W17446229 @default.
• W2022295532 cites W179818011 @default.
• W2022295532 cites W1818748679 @default.
• W2022295532 cites W1821840597 @default.
• W2022295532 cites W1958031853 @default.
• W2022295532 cites W1961043936 @default.
• W2022295532 cites W1968139214 @default.
• W2022295532 cites W1968511762 @default.
• W2022295532 cites W1972149163 @default.
• W2022295532 cites W1973858964 @default.
• W2022295532 cites W1977106721 @default.
• W2022295532 cites W1983683479 @default.
• W2022295532 cites W1990717510 @default.
• W2022295532 cites W1990989603 @default.
• W2022295532 cites W2003040518 @default.
• W2022295532 cites W2005484362 @default.
• W2022295532 cites W2007625577 @default.
• W2022295532 cites W2009820004 @default.
• W2022295532 cites W2012421057 @default.
• W2022295532 cites W2029275361 @default.
• W2022295532 cites W2039081691 @default.
• W2022295532 cites W2066397836 @default.
• W2022295532 cites W2075879868 @default.
• W2022295532 cites W2078832330 @default.
• W2022295532 cites W2089588876 @default.
• W2022295532 cites W2118636735 @default.
• W2022295532 cites W2119725374 @default.
• W2022295532 cites W2142366629 @default.
• W2022295532 cites W2146886804 @default.
• W2022295532 cites W2152098493 @default.
• W2022295532 cites W2153701698 @default.
• W2022295532 cites W2409057946 @default.
• W2022295532 cites W43827711 @default.
• W2022295532 cites W63830776 @default.
• W2022295532 doi "https://doi.org/10.1074/jbc.270.43.25584" @default.
• W2022295532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7592730" @default.
• W2022295532 hasPublicationYear "1995" @default.
• W2022295532 type Work @default.
• W2022295532 sameAs 2022295532 @default.
• W2022295532 citedByCount "72" @default.
• W2022295532 countsByYear W20222955322012 @default.
• W2022295532 countsByYear W20222955322013 @default.
• W2022295532 countsByYear W20222955322014 @default.
• W2022295532 countsByYear W20222955322015 @default.
• W2022295532 countsByYear W20222955322016 @default.
• W2022295532 countsByYear W20222955322017 @default.
• W2022295532 countsByYear W20222955322018 @default.
• W2022295532 countsByYear W20222955322019 @default.
• W2022295532 countsByYear W20222955322020 @default.
• W2022295532 countsByYear W20222955322021 @default.
• W2022295532 countsByYear W20222955322022 @default.
• W2022295532 countsByYear W20222955322023 @default.
• W2022295532 crossrefType "journal-article" @default.
• W2022295532 hasAuthorship W2022295532A5004616732 @default.
• W2022295532 hasAuthorship W2022295532A5031770387 @default.
• W2022295532 hasAuthorship W2022295532A5046740981 @default.
• W2022295532 hasAuthorship W2022295532A5070227595 @default.
• W2022295532 hasBestOaLocation W20222955321 @default.
• W2022295532 hasConcept C101762097 @default.
• W2022295532 hasConcept C104317684 @default.
• W2022295532 hasConcept C111936080 @default.
• W2022295532 hasConcept C150194340 @default.
• W2022295532 hasConcept C153911025 @default.
• W2022295532 hasConcept C161733203 @default.
• W2022295532 hasConcept C54355233 @default.
• W2022295532 hasConcept C58237431 @default.
• W2022295532 hasConcept C65888428 @default.
• W2022295532 hasConcept C86339819 @default.
• W2022295532 hasConcept C86803240 @default.
• W2022295532 hasConcept C91779695 @default.
• W2022295532 hasConceptScore W2022295532C101762097 @default.
• W2022295532 hasConceptScore W2022295532C104317684 @default.
• W2022295532 hasConceptScore W2022295532C111936080 @default.
• W2022295532 hasConceptScore W2022295532C150194340 @default.
• W2022295532 hasConceptScore W2022295532C153911025 @default.
• W2022295532 hasConceptScore W2022295532C161733203 @default.
• W2022295532 hasConceptScore W2022295532C54355233 @default.
• W2022295532 hasConceptScore W2022295532C58237431 @default.
• W2022295532 hasConceptScore W2022295532C65888428 @default.
• W2022295532 hasConceptScore W2022295532C86339819 @default.
• W2022295532 hasConceptScore W2022295532C86803240 @default.

• next