Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022307495> ?p ?o ?g. }
- W2022307495 endingPage "224" @default.
- W2022307495 startingPage "218" @default.
- W2022307495 abstract "Alveolar overdistension during mechanical ventilation causes leukocyte sequestration, leading to lung injury. However, underlying endothelial cell (EC) mechanisms are undefined. In a new approach, we exposed isolated blood-perfused rat lungs to high tidal volume ventilation (HV) for 2 h, then obtained fresh lung endothelial cells (FLEC) by immunosorting at 4 degrees C. Immunoblotting experiments indicated that as compared with FLEC derived from lungs ventilated at low volume (LV), HV markedly enhanced tyrosine phosphorylation (TyrP). The tyrosine kinase blocker, genistein, inhibited this response. HV also induced focal adhesion (FA) formation in FLEC, as detected by immunofluorescent aggregates of the alpha(v)beta(3) integrin that co-localized with aggregations of focal adhesion kinase (FAK). Immunoprecipitation and blotting experiments revealed that HV increased TyrP of the FA protein, paxillin. In addition, HV induced a paxillin-associated P-selectin expression on FLEC that was also inhibited by genistein. However, HV did not increase lung water. These results indicate that in HV, EC signaling in situ causes FA formation and induces TyrP-dependent P-selectin expression. These signaling mechanisms may promote leukocyte-mediated responses in HV." @default.
- W2022307495 created "2016-06-24" @default.
- W2022307495 creator A5041142136 @default.
- W2022307495 creator A5051246656 @default.
- W2022307495 creator A5053962419 @default.
- W2022307495 creator A5072655204 @default.
- W2022307495 creator A5076054649 @default.
- W2022307495 creator A5082030400 @default.
- W2022307495 creator A5088184017 @default.
- W2022307495 creator A5089054248 @default.
- W2022307495 date "2003-02-01" @default.
- W2022307495 modified "2023-10-18" @default.
- W2022307495 title "High Tidal Volume Ventilation Induces Proinflammatory Signaling in Rat Lung Endothelium" @default.
- W2022307495 cites W111774198 @default.
- W2022307495 cites W1488252042 @default.
- W2022307495 cites W1581533309 @default.
- W2022307495 cites W1625745398 @default.
- W2022307495 cites W1656644300 @default.
- W2022307495 cites W1746540096 @default.
- W2022307495 cites W1941414980 @default.
- W2022307495 cites W1965050416 @default.
- W2022307495 cites W1972277176 @default.
- W2022307495 cites W1978577250 @default.
- W2022307495 cites W1978985265 @default.
- W2022307495 cites W1990132839 @default.
- W2022307495 cites W1999063996 @default.
- W2022307495 cites W2000744097 @default.
- W2022307495 cites W2001622142 @default.
- W2022307495 cites W2004562001 @default.
- W2022307495 cites W2004680360 @default.
- W2022307495 cites W2010546098 @default.
- W2022307495 cites W2030841147 @default.
- W2022307495 cites W2063646489 @default.
- W2022307495 cites W2071584103 @default.
- W2022307495 cites W2072199945 @default.
- W2022307495 cites W2072339114 @default.
- W2022307495 cites W2072406547 @default.
- W2022307495 cites W2073623140 @default.
- W2022307495 cites W2079941180 @default.
- W2022307495 cites W2087650119 @default.
- W2022307495 cites W2094721974 @default.
- W2022307495 cites W2098400443 @default.
- W2022307495 cites W2099364812 @default.
- W2022307495 cites W2099764303 @default.
- W2022307495 cites W2107444275 @default.
- W2022307495 cites W2117013761 @default.
- W2022307495 cites W2133701478 @default.
- W2022307495 cites W2143626238 @default.
- W2022307495 cites W2151109915 @default.
- W2022307495 cites W2157020787 @default.
- W2022307495 cites W2162168130 @default.
- W2022307495 cites W2177430700 @default.
- W2022307495 cites W2314995980 @default.
- W2022307495 cites W2346035781 @default.
- W2022307495 cites W2460792408 @default.
- W2022307495 cites W4254300908 @default.
- W2022307495 doi "https://doi.org/10.1165/rcmb.4763" @default.
- W2022307495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12540489" @default.
- W2022307495 hasPublicationYear "2003" @default.
- W2022307495 type Work @default.
- W2022307495 sameAs 2022307495 @default.
- W2022307495 citedByCount "42" @default.
- W2022307495 countsByYear W20223074952012 @default.
- W2022307495 countsByYear W20223074952013 @default.
- W2022307495 countsByYear W20223074952014 @default.
- W2022307495 countsByYear W20223074952015 @default.
- W2022307495 countsByYear W20223074952016 @default.
- W2022307495 countsByYear W20223074952018 @default.
- W2022307495 countsByYear W20223074952019 @default.
- W2022307495 countsByYear W20223074952021 @default.
- W2022307495 countsByYear W20223074952022 @default.
- W2022307495 crossrefType "journal-article" @default.
- W2022307495 hasAuthorship W2022307495A5041142136 @default.
- W2022307495 hasAuthorship W2022307495A5051246656 @default.
- W2022307495 hasAuthorship W2022307495A5053962419 @default.
- W2022307495 hasAuthorship W2022307495A5072655204 @default.
- W2022307495 hasAuthorship W2022307495A5076054649 @default.
- W2022307495 hasAuthorship W2022307495A5082030400 @default.
- W2022307495 hasAuthorship W2022307495A5088184017 @default.
- W2022307495 hasAuthorship W2022307495A5089054248 @default.
- W2022307495 hasConcept C104317684 @default.
- W2022307495 hasConcept C116581196 @default.
- W2022307495 hasConcept C11960822 @default.
- W2022307495 hasConcept C126322002 @default.
- W2022307495 hasConcept C134018914 @default.
- W2022307495 hasConcept C151166631 @default.
- W2022307495 hasConcept C153911025 @default.
- W2022307495 hasConcept C164027704 @default.
- W2022307495 hasConcept C185592680 @default.
- W2022307495 hasConcept C203014093 @default.
- W2022307495 hasConcept C2776914184 @default.
- W2022307495 hasConcept C2777553839 @default.
- W2022307495 hasConcept C2777714996 @default.
- W2022307495 hasConcept C2778298627 @default.
- W2022307495 hasConcept C42362537 @default.
- W2022307495 hasConcept C55493867 @default.
- W2022307495 hasConcept C62478195 @default.
- W2022307495 hasConcept C71924100 @default.