FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022311963> ?p ?o ?g. }

• W2022311963 endingPage "154" @default.
• W2022311963 startingPage "148" @default.
• W2022311963 abstract "This study compared the effect of adding other cholesterol conjugates, which should incorporate into and increase sperm membrane fluidity at low temperatures thereby increasing cryosurvival. Ejaculates from each of four bulls were diluted to 120 million cells/ml in a Tris diluent and used in two experiments. Each experiment contained four treatments: No additive (control); 1.5mg CLC/120 million sperm (positive control); and 1.5mg cyclodextrin pre-loaded with cholestanol or desmosterol/120 million sperm. In the first experiment, fresh sperm were treated with cyclodextrins that were pre-loaded with cholesteryl conjugates and incubated for 15 min at 22 degrees C to allow incorporation of the conjugates. The percentages of motile sperm after exposure to solutions ranging from 0 to 1200 mOsm were used to evaluate the osmotic tolerance of the cells. The ability of treated sperm to bind to the bovine zona pellucida (ZP) and chicken egg perivitelline membrane (CEPM) was evaluated to determine if altering the sperm membrane affected the ability of sperm to bind to oocytes. In the final experiment, the cryosurvival rates of control and treated sperm were determined. Control and treated sperm were cryopreserved in a Tris diluent containing 10% egg yolk (EY) and 8% glycerol (final concentrations). Samples were thawed to determine the motility and ability of sperm to bind to the ZP and to CEPM using a CASA and epifluorescence microscope, respectively. Fresh sperm treated with CLC resulted in more binding to the ZP compared to all other treatments (P<0.05). No differences were observed between ZP and CEPM binding (P>0.05). The percentages of motile sperm were greater for fresh samples treated with cholesterol, cholestanol or desmosterol loaded cyclodextrin than control cells (P<0.05) when the sperm were exposed to anisosmotic conditions, and then returned to isosmolality. After cryopreservation the percentages of motile sperm and number of sperm binding to each CEPM were similar for sperm treated with CLC and cholestanol compared to sperm treated with desmosterol (P>0.05). All treatments provided greater motility and binding efficiency than control sperm (P<0.05). Therefore, adding cholesterol or cholestanol to bull sperm membranes improved cell cryosurvival." @default.
• W2022311963 created "2016-06-24" @default.
• W2022311963 creator A5008787556 @default.
• W2022311963 creator A5022570952 @default.
• W2022311963 creator A5024832703 @default.
• W2022311963 creator A5049364329 @default.
• W2022311963 creator A5090325236 @default.
• W2022311963 date "2010-04-01" @default.
• W2022311963 modified "2023-09-23" @default.
• W2022311963 title "Delivering cholesterol or cholestanol to bull sperm membranes improves cryosurvival" @default.
• W2022311963 cites W106002827 @default.
• W2022311963 cites W1966641759 @default.
• W2022311963 cites W1977094989 @default.
• W2022311963 cites W1980023194 @default.
• W2022311963 cites W1987898211 @default.
• W2022311963 cites W1990550372 @default.
• W2022311963 cites W1995242288 @default.
• W2022311963 cites W2005754620 @default.
• W2022311963 cites W2009475191 @default.
• W2022311963 cites W2019176296 @default.
• W2022311963 cites W2025585584 @default.
• W2022311963 cites W2027785265 @default.
• W2022311963 cites W2043793882 @default.
• W2022311963 cites W2059384421 @default.
• W2022311963 cites W2061424843 @default.
• W2022311963 cites W2067886138 @default.
• W2022311963 cites W2076844113 @default.
• W2022311963 cites W2084993968 @default.
• W2022311963 cites W2085155504 @default.
• W2022311963 cites W2086107609 @default.
• W2022311963 cites W2090334223 @default.
• W2022311963 cites W2121409037 @default.
• W2022311963 cites W2129586915 @default.
• W2022311963 cites W2130388575 @default.
• W2022311963 cites W2144486830 @default.
• W2022311963 cites W2165130521 @default.
• W2022311963 cites W2167691761 @default.
• W2022311963 cites W2170767880 @default.
• W2022311963 cites W2171930469 @default.
• W2022311963 cites W2418199106 @default.
• W2022311963 cites W2859718140 @default.
• W2022311963 doi "https://doi.org/10.1016/j.anireprosci.2009.08.002" @default.
• W2022311963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19733986" @default.
• W2022311963 hasPublicationYear "2010" @default.
• W2022311963 type Work @default.
• W2022311963 sameAs 2022311963 @default.
• W2022311963 citedByCount "53" @default.
• W2022311963 countsByYear W20223119632012 @default.
• W2022311963 countsByYear W20223119632013 @default.
• W2022311963 countsByYear W20223119632014 @default.
• W2022311963 countsByYear W20223119632015 @default.
• W2022311963 countsByYear W20223119632016 @default.
• W2022311963 countsByYear W20223119632017 @default.
• W2022311963 countsByYear W20223119632018 @default.
• W2022311963 countsByYear W20223119632019 @default.
• W2022311963 countsByYear W20223119632020 @default.
• W2022311963 countsByYear W20223119632021 @default.
• W2022311963 countsByYear W20223119632022 @default.
• W2022311963 crossrefType "journal-article" @default.
• W2022311963 hasAuthorship W2022311963A5008787556 @default.
• W2022311963 hasAuthorship W2022311963A5022570952 @default.
• W2022311963 hasAuthorship W2022311963A5024832703 @default.
• W2022311963 hasAuthorship W2022311963A5049364329 @default.
• W2022311963 hasAuthorship W2022311963A5090325236 @default.
• W2022311963 hasConcept C105702510 @default.
• W2022311963 hasConcept C16685009 @default.
• W2022311963 hasConcept C179933525 @default.
• W2022311963 hasConcept C185592680 @default.
• W2022311963 hasConcept C196843134 @default.
• W2022311963 hasConcept C2776010925 @default.
• W2022311963 hasConcept C2776690073 @default.
• W2022311963 hasConcept C2777085297 @default.
• W2022311963 hasConcept C2777632594 @default.
• W2022311963 hasConcept C2778093475 @default.
• W2022311963 hasConcept C2778163477 @default.
• W2022311963 hasConcept C2778718757 @default.
• W2022311963 hasConcept C2781087480 @default.
• W2022311963 hasConcept C55493867 @default.
• W2022311963 hasConcept C71924100 @default.
• W2022311963 hasConcept C86803240 @default.
• W2022311963 hasConcept C95444343 @default.
• W2022311963 hasConceptScore W2022311963C105702510 @default.
• W2022311963 hasConceptScore W2022311963C16685009 @default.
• W2022311963 hasConceptScore W2022311963C179933525 @default.
• W2022311963 hasConceptScore W2022311963C185592680 @default.
• W2022311963 hasConceptScore W2022311963C196843134 @default.
• W2022311963 hasConceptScore W2022311963C2776010925 @default.
• W2022311963 hasConceptScore W2022311963C2776690073 @default.
• W2022311963 hasConceptScore W2022311963C2777085297 @default.
• W2022311963 hasConceptScore W2022311963C2777632594 @default.
• W2022311963 hasConceptScore W2022311963C2778093475 @default.
• W2022311963 hasConceptScore W2022311963C2778163477 @default.
• W2022311963 hasConceptScore W2022311963C2778718757 @default.
• W2022311963 hasConceptScore W2022311963C2781087480 @default.
• W2022311963 hasConceptScore W2022311963C55493867 @default.
• W2022311963 hasConceptScore W2022311963C71924100 @default.
• W2022311963 hasConceptScore W2022311963C86803240 @default.
• W2022311963 hasConceptScore W2022311963C95444343 @default.

• next