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- W2022331431 abstract "Amounts of gp90MEL-14 molecules in various lymphocyte subpopulations were analyzed. The expression of gp90MEL-14 was rapidly up-regulated in the course of usual procedures for preparing single cell suspensions. On the other hand, the gp90MEL-14 expression was down-modulated after short term culture in the presence of PMA. Addition of NaN3 in the medium for preparing cell suspension was shown to stabilize the expression profile of the gp90MEL-14. By this simple improvement, we could successfully evaluate the precise amounts of gp90MEL-14 expressed on various lymphoid cells which appeared to reflect the expression pattern of gp90MEL-14in vivo. The proportion of MEL-14+ cells and amounts of gp90MEL-14 on the cells were clearly associated with the anatomical and functional distance from endothelial cells of HEV in peripheral lymph nodes. The present findings, thus, support the role of gp90MEL-14 molecules as homing receptors for HEV that was originally proposed by WEISSMAN and his colleagues." @default.
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- W2022331431 date "1992-11-01" @default.
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- W2022331431 title "Quantitative analysis of MEL-14 expression on various lymphocyte subpopulations" @default.
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- W2022331431 doi "https://doi.org/10.1016/s0171-2985(11)80256-1" @default.
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