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• W2022333403 abstract "<h3>Objective</h3> To study the effect of peritoneal fluid from women with (PF-E) and without (PF-C) endometriosis on P₄₅₀Arom expression in endometrial cells. <h3>Design</h3> Experimental study. <h3>Setting</h3> University research unit. <h3>Patient(s)</h3> Forty women of reproductive age with (n = 22) or without (control; n=18) endometriosis. <h3>Intervention(s)</h3> Peritoneal fluid and eutopic endometrial samples were obtained during surgery from women with (n = 13 and 9, respectively) and without (n = 4 and 14, respectively) endometriosis. <h3>Main Outcome Measure(s)</h3> Expression study for P₄₅₀Arom, steroid factor 1 (SF-1), chicken ovalbumin upstream transcription factor I (COUP-TFI), and COUP-TFII messenger RNA (reverse transcriptase–polymerase chain reacion) and/or protein (immunoblot) in isolated endometrial epithelial cells transfected or not with expression vector containing SF-1, COUP-TFI, or COUP-TFII complementary DNAs. <h3>Result(s)</h3> Basal messenger RNA and/or protein expression of P₄₅₀Arom and SF-1 were augmented in endometriosis, and that of COUP-TF was diminished. In control cells, (Bu)₂cAMP and PF-E increased P₄₅₀Arom and SF-1 expression (but not COUP-TF expression) in a dose-dependent way, an effect not observed with PF-C, adsorbed PF-E, or 10⁻⁵ M indomethacin. Transfected cells confirmed these results. Any treatments modified the studied molecules in endometriosis cells. <h3>Conclusion(s)</h3> These data indicate that molecules contained in PF-E favor an estrogenic microenvironment, suggesting a role in the etiopathogenesis of endometriosis enabling the survival, maintenance, and growth of endometrial implants in the ectopic locations." @default.
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• W2022333403 date "2010-12-01" @default.
• W2022333403 modified "2023-10-10" @default.
• W2022333403 title "P450Arom induction in isolated control endometrial cells by peritoneal fluid from women with endometriosis" @default.
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• W2022333403 doi "https://doi.org/10.1016/j.fertnstert.2010.03.036" @default.
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