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• W2022340022 endingPage "501" @default.
• W2022340022 startingPage "488" @default.
• W2022340022 abstract "We have previously demonstrated that Kir3.1 channels and Gβ1γ2 subunits initially interact in the endoplasmic reticulum (ER). To elucidate the role that anterograde protein trafficking pathways may play in the formation of these complexes, we used dominant negative (DN) mutants of the small G proteins Sar 1 and various compartment-specific Rabs which impede anterograde protein trafficking at different steps. Sar 1 H79G and Rab 1 S25N mutants efficiently blocked the plasma membrane trafficking of the Kir3.1/Kir3.4 complex however they did not block the Gβ1γ2/Kir3.1 interaction as measured using bioluminescence resonance energy transfer (BRET). This interaction was also insensitive to the presence of DN Rabs 2, 6, 8, and 11. These results confirm that Gβγ/Kir3 complexes form early during channel biosynthesis and trafficking. Using a combination of BRET, protein complementation assays and co-immunoprecipitation, we demonstrate that Gβ1-4 can interact with Kir3.1 in the absence of Kir3.4. Gβ5 does not directly interact with the channel but can still be co-immunoprecipated as part of a larger complex. The interaction between Gβ and Kir3.1 was selectively fostered by co-expression with different Gγ subunits. When Gγ1 or Gγ11 was co-expressed with eGFP-Gβ3 or eGFP-Gβ4, the interaction with the effector was lost. Kir3.2 was capable of interacting with Gβ1-3 and not Gβ4 or Gβ5. These interactions were again fostered by co-expression with Gγ and were also insensitive to DN Sar 1 or Rab 1. Taken together, our data show that these “precocious” channel/G protein interactions are specific and may have implications beyond their basic function in signalling events." @default.
• W2022340022 created "2016-06-24" @default.
• W2022340022 creator A5010381857 @default.
• W2022340022 creator A5043756020 @default.
• W2022340022 creator A5052328017 @default.
• W2022340022 creator A5067295524 @default.
• W2022340022 date "2009-04-01" @default.
• W2022340022 modified "2023-10-12" @default.
• W2022340022 title "Intracellular trafficking and assembly of specific Kir3 channel/G protein complexes" @default.
• W2022340022 cites W1504684037 @default.
• W2022340022 cites W1515479627 @default.
• W2022340022 cites W1524641925 @default.
• W2022340022 cites W1582905488 @default.
• W2022340022 cites W1595083713 @default.
• W2022340022 cites W1598669426 @default.
• W2022340022 cites W1956706703 @default.
• W2022340022 cites W1964871401 @default.
• W2022340022 cites W1967738476 @default.
• W2022340022 cites W1971777722 @default.
• W2022340022 cites W1973867786 @default.
• W2022340022 cites W1976149002 @default.
• W2022340022 cites W1977056558 @default.
• W2022340022 cites W1980891899 @default.
• W2022340022 cites W1983203888 @default.
• W2022340022 cites W1983399409 @default.
• W2022340022 cites W1990665465 @default.
• W2022340022 cites W2007149985 @default.
• W2022340022 cites W2008374286 @default.
• W2022340022 cites W2012043177 @default.
• W2022340022 cites W2013997965 @default.
• W2022340022 cites W2017689107 @default.
• W2022340022 cites W2023649595 @default.
• W2022340022 cites W2025837534 @default.
• W2022340022 cites W2027056512 @default.
• W2022340022 cites W2040478551 @default.
• W2022340022 cites W2040896718 @default.
• W2022340022 cites W2045254177 @default.
• W2022340022 cites W2046002448 @default.
• W2022340022 cites W2046722466 @default.
• W2022340022 cites W2056965979 @default.
• W2022340022 cites W2063069260 @default.
• W2022340022 cites W2067424234 @default.
• W2022340022 cites W2068095120 @default.
• W2022340022 cites W2068177438 @default.
• W2022340022 cites W2072237956 @default.
• W2022340022 cites W2073405845 @default.
• W2022340022 cites W2077401555 @default.
• W2022340022 cites W2078094267 @default.
• W2022340022 cites W2079302947 @default.
• W2022340022 cites W2080985839 @default.
• W2022340022 cites W2081666755 @default.
• W2022340022 cites W2086219908 @default.
• W2022340022 cites W2087151025 @default.
• W2022340022 cites W2087308250 @default.
• W2022340022 cites W2087886422 @default.
• W2022340022 cites W2089996434 @default.
• W2022340022 cites W2090614971 @default.
• W2022340022 cites W2091742123 @default.
• W2022340022 cites W2093106644 @default.
• W2022340022 cites W2095269345 @default.
• W2022340022 cites W2095353203 @default.
• W2022340022 cites W2105041061 @default.
• W2022340022 cites W2109731462 @default.
• W2022340022 cites W2113945079 @default.
• W2022340022 cites W2117138208 @default.
• W2022340022 cites W2119913776 @default.
• W2022340022 cites W2137314283 @default.
• W2022340022 cites W2140664204 @default.
• W2022340022 cites W2146674228 @default.
• W2022340022 cites W2148462082 @default.
• W2022340022 cites W2153506882 @default.
• W2022340022 cites W2162169295 @default.
• W2022340022 cites W2162243747 @default.
• W2022340022 cites W2164531655 @default.
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• W2022340022 cites W4249976428 @default.
• W2022340022 doi "https://doi.org/10.1016/j.cellsig.2008.11.011" @default.
• W2022340022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19135528" @default.
• W2022340022 hasPublicationYear "2009" @default.
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