FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022343229> ?p ?o ?g. }

• W2022343229 endingPage "496" @default.
• W2022343229 startingPage "487" @default.
• W2022343229 abstract "This study was designed to analyze and compare tissue preservation efficiency of acetone (AC), formaldehyde (FA) and paraformaldehyde (PFA) on cryosections. Brain, kidney, heart and liver tissue of adult Balb/c mice were fixed with either FA or PFA prior to cryosectioning, or fixed with AC alone immediately after cryosectioning. Hematoxylin and eosin staining showed that AC is a poor fixative in preserving the general tissue and cellular organization. PFA, and to a lesser extent FA, produced significantly better results. Another set of cryosections were further analyzed to test the properties of those fixatives to preserve proteins from specific cell structures. Cytokeratin filaments, F-actin filaments and nuclei were immunolabeled and examined using confocal microscopy. Results demonstrated that, overall, PFA is the best fixative tested. However, FA fixation gave poor results in preserving neuronal tissues. Immunofluorescence confirmed the inefficiency of AC fixation, after which no specific labelling of cytokeratin filaments was detectable. Nevertheless, actin filaments were detectable on AC-fixed samples, a finding that was supported by the quantification of fluorescein-phalloidin binding to F-actin. Overall, the data suggest that AC fixation is unacceptable for preservation of most samples, whereas FA and PFA fixation should be chosen according to the tissues and proteins to be studied." @default.
• W2022343229 created "2016-06-24" @default.
• W2022343229 creator A5062778222 @default.
• W2022343229 creator A5070844729 @default.
• W2022343229 creator A5079111087 @default.
• W2022343229 date "2006-12-01" @default.
• W2022343229 modified "2023-09-28" @default.
• W2022343229 title "A microscopic survey on the efficiency of well-known routine chemical fixatives on cryosections" @default.
• W2022343229 cites W2000199216 @default.
• W2022343229 cites W2012157299 @default.
• W2022343229 cites W2014821819 @default.
• W2022343229 cites W2016008120 @default.
• W2022343229 cites W2020525598 @default.
• W2022343229 cites W2090855399 @default.
• W2022343229 cites W2127676040 @default.
• W2022343229 cites W2128927944 @default.
• W2022343229 cites W2150398616 @default.
• W2022343229 cites W2162765095 @default.
• W2022343229 cites W2168999755 @default.
• W2022343229 cites W2170711285 @default.
• W2022343229 cites W4238766851 @default.
• W2022343229 cites W4301221324 @default.
• W2022343229 cites W4322701856 @default.
• W2022343229 cites W50693580 @default.
• W2022343229 doi "https://doi.org/10.1016/j.acthis.2006.05.008" @default.
• W2022343229 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16950501" @default.
• W2022343229 hasPublicationYear "2006" @default.
• W2022343229 type Work @default.
• W2022343229 sameAs 2022343229 @default.
• W2022343229 citedByCount "17" @default.
• W2022343229 countsByYear W20223432292012 @default.
• W2022343229 countsByYear W20223432292014 @default.
• W2022343229 countsByYear W20223432292015 @default.
• W2022343229 countsByYear W20223432292017 @default.
• W2022343229 countsByYear W20223432292019 @default.
• W2022343229 countsByYear W20223432292020 @default.
• W2022343229 countsByYear W20223432292022 @default.
• W2022343229 crossrefType "journal-article" @default.
• W2022343229 hasAuthorship W2022343229A5062778222 @default.
• W2022343229 hasAuthorship W2022343229A5070844729 @default.
• W2022343229 hasAuthorship W2022343229A5079111087 @default.
• W2022343229 hasConcept C104317684 @default.
• W2022343229 hasConcept C142669718 @default.
• W2022343229 hasConcept C142724271 @default.
• W2022343229 hasConcept C146249460 @default.
• W2022343229 hasConcept C1491633281 @default.
• W2022343229 hasConcept C178790620 @default.
• W2022343229 hasConcept C185592680 @default.
• W2022343229 hasConcept C203014093 @default.
• W2022343229 hasConcept C204232928 @default.
• W2022343229 hasConcept C2776861759 @default.
• W2022343229 hasConcept C2778723968 @default.
• W2022343229 hasConcept C2779012181 @default.
• W2022343229 hasConcept C2779178360 @default.
• W2022343229 hasConcept C2780848204 @default.
• W2022343229 hasConcept C54355233 @default.
• W2022343229 hasConcept C55493867 @default.
• W2022343229 hasConcept C71924100 @default.
• W2022343229 hasConcept C74864618 @default.
• W2022343229 hasConcept C86803240 @default.
• W2022343229 hasConcept C95444343 @default.
• W2022343229 hasConceptScore W2022343229C104317684 @default.
• W2022343229 hasConceptScore W2022343229C142669718 @default.
• W2022343229 hasConceptScore W2022343229C142724271 @default.
• W2022343229 hasConceptScore W2022343229C146249460 @default.
• W2022343229 hasConceptScore W2022343229C1491633281 @default.
• W2022343229 hasConceptScore W2022343229C178790620 @default.
• W2022343229 hasConceptScore W2022343229C185592680 @default.
• W2022343229 hasConceptScore W2022343229C203014093 @default.
• W2022343229 hasConceptScore W2022343229C204232928 @default.
• W2022343229 hasConceptScore W2022343229C2776861759 @default.
• W2022343229 hasConceptScore W2022343229C2778723968 @default.
• W2022343229 hasConceptScore W2022343229C2779012181 @default.
• W2022343229 hasConceptScore W2022343229C2779178360 @default.
• W2022343229 hasConceptScore W2022343229C2780848204 @default.
• W2022343229 hasConceptScore W2022343229C54355233 @default.
• W2022343229 hasConceptScore W2022343229C55493867 @default.
• W2022343229 hasConceptScore W2022343229C71924100 @default.
• W2022343229 hasConceptScore W2022343229C74864618 @default.
• W2022343229 hasConceptScore W2022343229C86803240 @default.
• W2022343229 hasConceptScore W2022343229C95444343 @default.
• W2022343229 hasIssue "6" @default.
• W2022343229 hasLocation W20223432291 @default.
• W2022343229 hasLocation W20223432292 @default.
• W2022343229 hasOpenAccess W2022343229 @default.
• W2022343229 hasPrimaryLocation W20223432291 @default.
• W2022343229 hasRelatedWork W1838940249 @default.
• W2022343229 hasRelatedWork W1985303502 @default.
• W2022343229 hasRelatedWork W1996034068 @default.
• W2022343229 hasRelatedWork W2021422745 @default.
• W2022343229 hasRelatedWork W2022343229 @default.
• W2022343229 hasRelatedWork W2063644207 @default.
• W2022343229 hasRelatedWork W2075604928 @default.
• W2022343229 hasRelatedWork W2086400449 @default.
• W2022343229 hasRelatedWork W2992687774 @default.
• W2022343229 hasRelatedWork W58684494 @default.
• W2022343229 hasVolume "108" @default.
• W2022343229 isParatext "false" @default.

• next