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• W2022349609 abstract "Mitochondria from the yeast S. cerevisiae have been shown to be particularly suited for cryobiological studies, since they may be frozen and thawed without detectable injury to either structure or function. Mitochondria are frozen in the usual isolation medium containing mannitol and serum albumin, and no penetrating cryoprotective agent such as dimethyl sulfoxide or glycerol is required. Yeast cells have been stored at 4°C for at least 9 days, and isolated mitochondria for 30 days or more, in liquid N2 without appreciable change in mitochondrial Qo2, ADP:O, or adenosine diphosphate-linked respiratory control. Stored mitochondria retain normal morphology and capacity for adenine nucleotide translocation. Respiratory control has been preserved for as long as 53 days. Improved isolation methods together with these storage techniques have permitted study of intact yeast mitochondria with a facility virtually unattainable with mitochondria from other cell types. Mitochondria isolated from a yeast mutant with deficient aerobic energy metabolism exhibit increased lability during freezing and thawing. Both Qo2 and respiratory control are greatly reduced." @default.
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• W2022349609 date "1970-03-01" @default.
• W2022349609 modified "2023-09-26" @default.
• W2022349609 title "Cryobiological studies of yeast mitochondria" @default.
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• W2022349609 doi "https://doi.org/10.1016/s0011-2240(70)80094-3" @default.
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