Matches in SemOpenAlex for { <https://semopenalex.org/work/W2022350385> ?p ?o ?g. }
- W2022350385 endingPage "859" @default.
- W2022350385 startingPage "849" @default.
- W2022350385 abstract "Intracellular pH (pHi) dynamics regulates diverse cellular processes, including remodeling of focal adhesions. We now report that focal adhesion kinase (FAK), a key regulator of focal adhesion remodeling, is a pH sensor responding to physiological changes in pH. The initial step in FAK activation is autophosphorylation of Tyr397, which increased with higher pHi. We used a genetically encoded biosensor to show increased pH at focal adhesions as they mature during cell spreading. We also show that cells with reduced pHi had attenuated FAK-pY397 as well as defective cell spreading and focal adhesions. Mutagenesis studies indicated FAK-His58 is critical for pH sensing and molecular dynamics simulations suggested a model in which His58 deprotonation drives conformational changes that may modulate accessibility of Tyr397 for autophosphorylation. Expression of FAK-H58A in fibroblasts was sufficient to restore defective autophosphorylation and cell spreading at low pHi. These data are relevant to understanding cancer metastasis, which is dependent on increased pHi and FAK activity." @default.
- W2022350385 created "2016-06-24" @default.
- W2022350385 creator A5001224002 @default.
- W2022350385 creator A5042219792 @default.
- W2022350385 creator A5052272649 @default.
- W2022350385 creator A5052737968 @default.
- W2022350385 creator A5078551237 @default.
- W2022350385 date "2013-09-16" @default.
- W2022350385 modified "2023-10-12" @default.
- W2022350385 title "pH sensing by FAK-His58 regulates focal adhesion remodeling" @default.
- W2022350385 cites W1577573926 @default.
- W2022350385 cites W1594588487 @default.
- W2022350385 cites W1845277443 @default.
- W2022350385 cites W1904209158 @default.
- W2022350385 cites W1929023701 @default.
- W2022350385 cites W1964020745 @default.
- W2022350385 cites W1970516036 @default.
- W2022350385 cites W1976499671 @default.
- W2022350385 cites W1976965279 @default.
- W2022350385 cites W1977426071 @default.
- W2022350385 cites W1988194926 @default.
- W2022350385 cites W1988664472 @default.
- W2022350385 cites W2003698382 @default.
- W2022350385 cites W2015599538 @default.
- W2022350385 cites W2021832765 @default.
- W2022350385 cites W2025787878 @default.
- W2022350385 cites W2026327508 @default.
- W2022350385 cites W2026428775 @default.
- W2022350385 cites W2028691987 @default.
- W2022350385 cites W2028936118 @default.
- W2022350385 cites W2029667189 @default.
- W2022350385 cites W2041200273 @default.
- W2022350385 cites W2048209624 @default.
- W2022350385 cites W2051381895 @default.
- W2022350385 cites W2059492700 @default.
- W2022350385 cites W2060315853 @default.
- W2022350385 cites W2065376967 @default.
- W2022350385 cites W2068050569 @default.
- W2022350385 cites W2068752808 @default.
- W2022350385 cites W2076352550 @default.
- W2022350385 cites W2078450213 @default.
- W2022350385 cites W2094258184 @default.
- W2022350385 cites W2095100212 @default.
- W2022350385 cites W2105766219 @default.
- W2022350385 cites W2113551776 @default.
- W2022350385 cites W2126107464 @default.
- W2022350385 cites W2129153475 @default.
- W2022350385 cites W2135604793 @default.
- W2022350385 cites W2148541235 @default.
- W2022350385 cites W2148814198 @default.
- W2022350385 cites W2149146087 @default.
- W2022350385 cites W2153448114 @default.
- W2022350385 cites W2164160141 @default.
- W2022350385 cites W2171235356 @default.
- W2022350385 cites W2172260616 @default.
- W2022350385 cites W2295313459 @default.
- W2022350385 cites W9261019 @default.
- W2022350385 doi "https://doi.org/10.1083/jcb.201302131" @default.
- W2022350385 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3776353" @default.
- W2022350385 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24043700" @default.
- W2022350385 hasPublicationYear "2013" @default.
- W2022350385 type Work @default.
- W2022350385 sameAs 2022350385 @default.
- W2022350385 citedByCount "75" @default.
- W2022350385 countsByYear W20223503852013 @default.
- W2022350385 countsByYear W20223503852014 @default.
- W2022350385 countsByYear W20223503852015 @default.
- W2022350385 countsByYear W20223503852016 @default.
- W2022350385 countsByYear W20223503852017 @default.
- W2022350385 countsByYear W20223503852018 @default.
- W2022350385 countsByYear W20223503852019 @default.
- W2022350385 countsByYear W20223503852020 @default.
- W2022350385 countsByYear W20223503852021 @default.
- W2022350385 countsByYear W20223503852022 @default.
- W2022350385 countsByYear W20223503852023 @default.
- W2022350385 crossrefType "journal-article" @default.
- W2022350385 hasAuthorship W2022350385A5001224002 @default.
- W2022350385 hasAuthorship W2022350385A5042219792 @default.
- W2022350385 hasAuthorship W2022350385A5052272649 @default.
- W2022350385 hasAuthorship W2022350385A5052737968 @default.
- W2022350385 hasAuthorship W2022350385A5078551237 @default.
- W2022350385 hasBestOaLocation W20223503851 @default.
- W2022350385 hasConcept C11960822 @default.
- W2022350385 hasConcept C12554922 @default.
- W2022350385 hasConcept C137738243 @default.
- W2022350385 hasConcept C1491633281 @default.
- W2022350385 hasConcept C150109051 @default.
- W2022350385 hasConcept C151166631 @default.
- W2022350385 hasConcept C178790620 @default.
- W2022350385 hasConcept C185592680 @default.
- W2022350385 hasConcept C55493867 @default.
- W2022350385 hasConcept C79879829 @default.
- W2022350385 hasConcept C84416704 @default.
- W2022350385 hasConcept C85789140 @default.
- W2022350385 hasConcept C86803240 @default.
- W2022350385 hasConcept C95444343 @default.
- W2022350385 hasConcept C97029542 @default.
- W2022350385 hasConceptScore W2022350385C11960822 @default.