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- W2022354423 abstract "The a.c. polarographic behaviour of methotrexate (MTX) was studied and the nature of the process taking place at the dropping mercury electrode was elucidated. Both the molecule and its reduced product appeared to be absorbed at the surface of the electrode. The observed electrochemical behaviour was in close agreement with theoretical predictions for an absorbed species which is reversibly reduced. This permitted a very sensitive and selective determination of MTX in serum samples by phase-selective a.c. polarography. The method, which involves a very simple and rapid previous clean-up procedure, has a limit of detection of 3.0 × 10−7 M in serum and the results compare well with those obtained by liquid chromatography with oxidative amperometric detection (LC-ED) at +1.1 V. Both methods form an interesting alternative to others previously reported for monitoring MTX in cancer patients under treatment with high doses of the drug." @default.
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- W2022354423 date "1990-01-01" @default.
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- W2022354423 title "Phase-selective alternating current polarographic assay of methotrexate in human serum" @default.
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- W2022354423 doi "https://doi.org/10.1016/s0003-2670(00)83489-3" @default.
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