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• W2022357228 abstract "<i>Background:</i> Fetal neuroendocrine maturation in late gestation is critical for maintenance of fetal homeostasis, growth, and readiness for birth. Sheep express estrogen receptors (ERs) in various brain regions. However, little is known about the regulation of ER-α and ER-β in the ovine brain prenatally. <i>Objective:</i> The present study was designed to test the hypothesis that the expression of ER is influenced by circulating estrogens in the late-gestation sheep fetus. <i>Methods:</i> Six chronically-catheterized twin fetal sheep were treated with vehicle or the ER blocker ICI 182,780 i.c.v. (0.25 μg/day). Fetuses were sacrificed 6–14 days after surgery and start of infusion. Brain regions were rapidly isolated and snap-frozen for later extraction of mRNA and protein. ER-α and ER-β mRNA was measured using real-time PCR and protein was measured using Western blot. <i>Results:</i> Treatment with ICI 182,780 increased ER-α mRNA, especially in cerebellum and hippocampus. There were no changes in ER-α protein and no changes in ER-β at either the mRNA or protein level. <i>Conclusion:</i> Expression of ER-α is influenced by endogenous estrogens in the ovine fetal brain." @default.
• W2022357228 created "2016-06-24" @default.
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• W2022357228 date "2009-01-01" @default.
• W2022357228 modified "2023-10-17" @default.
• W2022357228 title "Blockade of Estrogen Action Upregulates Estrogen Receptor-α mRNA in the Fetal Brain" @default.
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• W2022357228 doi "https://doi.org/10.1159/000208793" @default.
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