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- W2022368410 endingPage "e1000169" @default.
- W2022368410 startingPage "e1000169" @default.
- W2022368410 abstract "Hematogenous dissemination is important for infection by many bacterial pathogens, but is poorly understood because of the inability to directly observe this process in living hosts at the single cell level. All disseminating pathogens must tether to the host endothelium despite significant shear forces caused by blood flow. However, the molecules that mediate tethering interactions have not been identified for any bacterial pathogen except E. coli, which tethers to host cells via a specialized pillus structure that is not found in many pathogens. Furthermore, the mechanisms underlying tethering have never been examined in living hosts. We recently engineered a fluorescent strain of Borrelia burgdorferi, the Lyme disease pathogen, and visualized its dissemination from the microvasculature of living mice using intravital microscopy. We found that dissemination was a multistage process that included tethering, dragging, stationary adhesion and extravasation. In the study described here, we used quantitative real-time intravital microscopy to investigate the mechanistic features of the vascular interaction stage of B. burgdorferi dissemination. We found that tethering and dragging interactions were mechanistically distinct from stationary adhesion, and constituted the rate-limiting initiation step of microvascular interactions. Surprisingly, initiation was mediated by host Fn and GAGs, and the Fn- and GAG-interacting B. burgdorferi protein BBK32. Initiation was also strongly inhibited by the low molecular weight clinical heparin dalteparin. These findings indicate that the initiation of spirochete microvascular interactions is dependent on host ligands known to interact in vitro with numerous other bacterial pathogens. This conclusion raises the intriguing possibility that fibronectin and GAG interactions might be a general feature of hematogenous dissemination by other pathogens." @default.
- W2022368410 created "2016-06-24" @default.
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- W2022368410 date "2008-10-03" @default.
- W2022368410 modified "2023-10-18" @default.
- W2022368410 title "Molecular Mechanisms Involved in Vascular Interactions of the Lyme Disease Pathogen in a Living Host" @default.
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- W2022368410 cites W1598181676 @default.
- W2022368410 cites W1666259683 @default.
- W2022368410 cites W1818745376 @default.
- W2022368410 cites W182113194 @default.
- W2022368410 cites W1884700299 @default.
- W2022368410 cites W1890231854 @default.
- W2022368410 cites W1964571929 @default.
- W2022368410 cites W1968090144 @default.
- W2022368410 cites W1972167942 @default.
- W2022368410 cites W1976117629 @default.
- W2022368410 cites W1979887297 @default.
- W2022368410 cites W1980287996 @default.
- W2022368410 cites W1982702686 @default.
- W2022368410 cites W1987104843 @default.
- W2022368410 cites W1993077446 @default.
- W2022368410 cites W1993283779 @default.
- W2022368410 cites W1993607801 @default.
- W2022368410 cites W1995781008 @default.
- W2022368410 cites W2007245268 @default.
- W2022368410 cites W2008639090 @default.
- W2022368410 cites W2017677880 @default.
- W2022368410 cites W2020645507 @default.
- W2022368410 cites W2021036513 @default.
- W2022368410 cites W2022013307 @default.
- W2022368410 cites W2029103031 @default.
- W2022368410 cites W2031556190 @default.
- W2022368410 cites W2035273924 @default.
- W2022368410 cites W2040595568 @default.
- W2022368410 cites W2049752634 @default.
- W2022368410 cites W2054115369 @default.
- W2022368410 cites W2055807983 @default.
- W2022368410 cites W2057463285 @default.
- W2022368410 cites W2058619745 @default.
- W2022368410 cites W2068259161 @default.
- W2022368410 cites W2070105244 @default.
- W2022368410 cites W2074605133 @default.
- W2022368410 cites W2077024798 @default.
- W2022368410 cites W2094083548 @default.
- W2022368410 cites W2097025955 @default.
- W2022368410 cites W2105115586 @default.
- W2022368410 cites W2109874283 @default.
- W2022368410 cites W2112509531 @default.
- W2022368410 cites W2114228461 @default.
- W2022368410 cites W2114574473 @default.
- W2022368410 cites W2116073343 @default.
- W2022368410 cites W2116553639 @default.
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- W2022368410 cites W2122107835 @default.
- W2022368410 cites W2123757128 @default.
- W2022368410 cites W2128432942 @default.
- W2022368410 cites W2128469158 @default.
- W2022368410 cites W2129279189 @default.
- W2022368410 cites W2131606068 @default.
- W2022368410 cites W2133662578 @default.
- W2022368410 cites W2141037678 @default.
- W2022368410 cites W2142369367 @default.
- W2022368410 cites W2143679420 @default.
- W2022368410 cites W2146361892 @default.
- W2022368410 cites W2146745552 @default.
- W2022368410 cites W2153219926 @default.
- W2022368410 cites W2157801459 @default.
- W2022368410 cites W2158809046 @default.
- W2022368410 cites W2162114252 @default.
- W2022368410 cites W2163003258 @default.
- W2022368410 cites W2164808173 @default.
- W2022368410 cites W2169271627 @default.
- W2022368410 cites W2171980738 @default.
- W2022368410 cites W2172106052 @default.
- W2022368410 cites W2369907359 @default.
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- W2022368410 doi "https://doi.org/10.1371/journal.ppat.1000169" @default.
- W2022368410 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2542414" @default.
- W2022368410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18833295" @default.
- W2022368410 hasPublicationYear "2008" @default.
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