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• W2022370671 abstract "We previously identified a cross-tick species conserved tick feeding stimuli responsive Amblyomma americanum (Aam) AV422 gene. This study demonstrates that AamAV422 belongs to a novel group of arthropod proteins that is characterized by 14 cysteine amino acid residues: C(23)-X7/9-C(33)-X23/24-C(58)-X8-C(67)-X7-C(75)-X23-C(99)-X15-C(115)-X10-C(126)-X24/25/33-C(150)C(151)-X7-C(159)-X8-C(168)-X23/24-C(192)-X9/10-C(202) predicted to form seven disulfide bonds. We show that AamAV422 protein is a ubiquitously expressed protein that is injected into the host within the first 24h of the tick attaching onto the host as revealed by Western blotting analyses of recombinant (r)AamAV422, tick saliva and dissected tick organ protein extracts using antibodies to 24 and 48 h tick saliva proteins. Native AamAV422 is apparently involved with mediating tick anti-hemostasis and anti-complement functions in that rAamAV422 delayed plasma clotting time in a dose responsive manner by up to ≈ 160 s, prevented platelet aggregation by up to ≈ 16% and caused ≈ 24% reduction in production of terminal complement complexes. Target validation analysis revealed that rAamAV422 is a potential candidate for a cocktail or multivalent tick vaccine preparation in that RNA interference (RNAi)-mediated silencing of AamAV422 mRNA caused a statistically significant (≈ 44%) reduction in tick engorgement weights, which is proxy for amounts of ingested blood. We speculate that AamAV422 is a potential target antigen for development of the highly desired universal tick vaccine in that consistent with high conservation among ticks, antibodies to 24h Ixodes scapularis tick saliva proteins specifically bound rAamAV422. We discuss data in this study in the context of advancing the biology of tick feeding physiology and discovery of potential target antigens for tick vaccine development." @default.
• W2022370671 created "2016-06-24" @default.
• W2022370671 creator A5021621194 @default.
• W2022370671 creator A5034157163 @default.
• W2022370671 creator A5088838774 @default.
• W2022370671 date "2013-05-01" @default.
• W2022370671 modified "2023-10-18" @default.
• W2022370671 title "Deorphanization and target validation of cross-tick species conserved novel Amblyomma americanum tick saliva protein" @default.
• W2022370671 cites W141821869 @default.
• W2022370671 cites W1645672333 @default.
• W2022370671 cites W1933888663 @default.
• W2022370671 cites W1962928044 @default.
• W2022370671 cites W1963916236 @default.
• W2022370671 cites W1964205581 @default.
• W2022370671 cites W1964681224 @default.
• W2022370671 cites W1967152825 @default.
• W2022370671 cites W1968768356 @default.
• W2022370671 cites W1979083993 @default.
• W2022370671 cites W1979440482 @default.
• W2022370671 cites W1981816138 @default.
• W2022370671 cites W1985407614 @default.
• W2022370671 cites W1988031662 @default.
• W2022370671 cites W1988365368 @default.
• W2022370671 cites W1988903086 @default.
• W2022370671 cites W1993291292 @default.
• W2022370671 cites W2005605475 @default.
• W2022370671 cites W2007462084 @default.
• W2022370671 cites W2009038320 @default.
• W2022370671 cites W2010252410 @default.
• W2022370671 cites W2015700840 @default.
• W2022370671 cites W2026185192 @default.
• W2022370671 cites W2026684630 @default.
• W2022370671 cites W2027546867 @default.
• W2022370671 cites W2028592328 @default.
• W2022370671 cites W2031549439 @default.
• W2022370671 cites W2033374283 @default.
• W2022370671 cites W2033545687 @default.
• W2022370671 cites W2034000618 @default.
• W2022370671 cites W2037877670 @default.
• W2022370671 cites W2043074836 @default.
• W2022370671 cites W2043568569 @default.
• W2022370671 cites W2045825469 @default.
• W2022370671 cites W2046011346 @default.
• W2022370671 cites W2048354057 @default.
• W2022370671 cites W2051463187 @default.
• W2022370671 cites W2062637402 @default.
• W2022370671 cites W2063377311 @default.
• W2022370671 cites W2067044289 @default.
• W2022370671 cites W2072075496 @default.
• W2022370671 cites W2072523200 @default.
• W2022370671 cites W2072708496 @default.
• W2022370671 cites W2073032227 @default.
• W2022370671 cites W2074551732 @default.
• W2022370671 cites W2077162746 @default.
• W2022370671 cites W2078480560 @default.
• W2022370671 cites W2079211220 @default.
• W2022370671 cites W2080072877 @default.
• W2022370671 cites W2084146420 @default.
• W2022370671 cites W2087056263 @default.
• W2022370671 cites W2092172266 @default.
• W2022370671 cites W2093632324 @default.
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• W2022370671 cites W2097903143 @default.
• W2022370671 cites W2103355691 @default.
• W2022370671 cites W2112648191 @default.
• W2022370671 cites W2121197587 @default.
• W2022370671 cites W2131824543 @default.
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• W2022370671 cites W2164769915 @default.
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• W2022370671 doi "https://doi.org/10.1016/j.ijpara.2012.12.012" @default.
• W2022370671 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4058329" @default.
• W2022370671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23428900" @default.
• W2022370671 hasPublicationYear "2013" @default.
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